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Fusarium tupiense sp nov., a member of the Gibberella fujikuroi complex that causes mango malformation in Brazil

机译:Fusarium tupiense sp nov。,Gibberella fujikuroi复合体的成员,导致巴西的芒果畸形

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摘要

Fusarium tupiense, the main causal agent of mango malformation in Brazil, is described through a combination of morphological, biological and molecular markers. This new species belongs to the Gibberella fujikuroi species complex (GFSC) and has an anamorph morphologically similar to Fusarium mangiferae and F. sterilihyphosum. F. tupiense can be differentiated from other species in the G. fujikuroi species complex on the basis of sexual crosses, amplified fragment length polymorphism (AFLP) markers and partial sequences of the tef1 and tub2 genes. Female fertility for field isolates of F. tupiense appears to be low. PCR with primers specific for the mating type (MAT) alleles and sexual crosses identified this species as heterothallic with two idiomorphs. Female-fertile tester strains were developed for the identification of field strains of this species through sexual crosses.
机译:番茄镰刀菌是巴西芒果畸形的主要病因,通过形态,生物学和分子标记的组合来描述。该新物种属于富士赤霉菌种复合体(GFSC),其形态上与镰刀镰刀菌和无菌镰刀菌相似。可以根据性杂交,扩增的片段长度多态性(AFLP)标记以及tef1和tub2基因的部分序列,将F. tupiense与G. fujikuroi物种群中的其他物种区分开。野豌豆田间分离株的雌性育性似乎很低。用对交配型(MAT)等位基因和性杂交有特异性的引物进行PCR,鉴定该物种为具有两个独特型的杂种。为了通过性杂交鉴定该物种的田间菌株,开发了雌性可育测试菌株。

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