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首页> 外文期刊>Molecular reproduction and development >Porcine oocyte activation induced by a cytosolic sperm factor
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Porcine oocyte activation induced by a cytosolic sperm factor

机译：胞质精子因子诱导的猪卵母细胞活化

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It is not known how the fertilizing sperm elicits the release of Ca2+ from the oocyte's intracellular stores. We investigated whether a crude extract isolated from boar sperm could induce the Ca2+ release and trigger subsequent early and late activation events upon injection into matured porcine oocytes. The sperm extract induced an immediate rise in the intracellular free Ca2+ concentration in all oocytes tested, which was followed by repetitive Ca2+ transients in 11 out of 14 oocytes. Heat or trypsin treatment of the extract totally abolished the Ca2+ releasing activity of the sperm factor. The injected oocytes showed cortical granule exocytosis, they resumed meiosis and entered first interphase: pronuclei were formed in 89.2% (132/148) of the cases. Pronuclear formation was accompanied by the appearance of a new 22 kDa protein as normally seen at fertilization. Of the successfully injected oocytes 51.7% (105/203) cleaved and 2.0% (4/203) developed to the blastocyst stage after being cultured for 7 days in NCSU 23 medium. Injection of the carrier medium could not trigger these changes. The results indicate that the sperm might activate porcine oocytes by introducing a soluble factor into the oocyte's cytoplasm after gamete fusion. Mel. Reprod. Dev. 57:290-295, 2000.
机译：尚不清楚受精的精子如何引起卵母细胞的细胞内存储中Ca2 +的释放。我们调查了从公猪精子中分离的粗提物是否可以诱导Ca2 +释放并在注入成熟的猪卵母细胞后触发随后的早期和晚期活化事件。精子提取物在所有测试的卵母细胞中引起细胞内游离Ca2 +浓度的立即升高，然后在14个卵母细胞中的11个卵母细胞中重复发生Ca2 +瞬变。提取物的加热或胰蛋白酶处理完全消除了精子因子的Ca2 +释放活性。注射的卵母细胞显示出皮质颗粒的胞吐作用，它们恢复了减数分裂并进入了第一个中间期：在89.2％（132/148）的病例中形成了原核。如在受精时通常所见，前核的形成伴随着新的22 kDa蛋白的出现。在NCSU 23培养基中培养7天后，成功注射的卵母细胞中有51.7％（105/203）裂解，有2.0％（4/203）发育为胚泡期。注入载体介质不会触发这些变化。结果表明，配子融合后，精子可能通过将可溶性因子引入卵母细胞的细胞质中来激活猪卵母细胞。梅尔责备。开发人员57：290-295，2000。

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