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Species composition of endophytic fungi in Amaranthus hybridus leaves, petioles, stems, and roots

机译:mar菜的叶,叶柄,茎和根内生真菌的种类组成

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The objectives of this study were to identify and quantify fungi in asymptomatic, 5-mo-old Amaranthus hybridus plants grown in cultivated plots and in a greenhouse, and determine the species composition in different plant parts. Plants were sampled in South Africa from a plot at Potchefstroom in 1997, from two plots at Bloemfontein in 1997, and from one plot at Bloemfontein in 1996. Leaves, petioles, roots, stems, and seeds were collected, surface disinfested, and small sections were placed on corn-meal agar; seeds were nut sectioned (9160 isolation attempts). All fungal colonies growing from the sections/seeds were transferred from colony margins onto separate agar plates and identified. Differences in species composition were found among plant parts (P < 0.001). The most common species isolated from leaves and petioles at both sites and both years were species of the Alternaria tenuissima group. Fungal genera other than Alternaria predominated in the roots with pronounced differences between the two sites. Isolation frequencies from stems and seeds were low This paper identifies the endophytic fungi found in A. hybridus and sets the stage for further studies dealing with these fungi. [References: 41]
机译:这项研究的目的是鉴定和定量在栽培地和温室中生长的无症状,5个月大的A菜杂种植物中的真菌,并确定不同植物部分的物种组成。南非从1997年在Potchefstroom的一个地块,1997年在布隆方丹的两个地块,以及1996年在布隆方丹的一个地块上对南非进行了采样。收集了叶,叶柄,根,茎和种子,对表面进行了除虫和小切片处理放在玉米粉琼脂上;将种子切成坚果(9160次隔离尝试)。从切片/种子生长的所有真菌菌落都从菌落边缘转移到单独的琼脂平板上并进行鉴定。发现植物部分之间的物种组成存在差异(P <0.001)。从这两个地点和两个年份的叶和叶柄中分离出的最常见物种是tenternaria tenuissima组的物种。除链格孢属以外的真菌属在根中占主导地位,两个部位之间存在明显差异。茎和种子的分离频率很低本文鉴定了杂种曲霉中发现的内生真菌,并为进一步研究这些真菌奠定了基础。 [参考:41]

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