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A molecular approach for the diagnosis of wood rotting disease in desert citrus

机译：诊断沙漠柑橘木材腐烂病的分子方法

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Brown heartwood rot is an increasingly important problem in southwestern deserts, affecting numerous native tree species, and commercial lemon production. Two fungal pathogens, Coniophora eremophila and Antrodia sinuosa, have been identified as causal agents of brown heartwood rot in citrus. Development of effective disease management strategies relies on the ability to readily identify and distinguish between the pathogens; therefore we have developed molecular markers as an alternative to morphometric methods to differentiate these two economically important pathogens. Random amplified polymorphic DNA analysis identified four primers that revealed fungal polymorphisms. Polymorphic DNA fragments were cloned, sequenced, and used to create a 700 bp sequence characterized amplified region (SCAR) in C. eremophila isolates using sequence-specific primers, Conio7 and Conio8. Three fragments (300, 650, and 1000 bp) were amplified with Conio7 and Conio8 in A. sinuosa isolates. A rapid, small scale method for DNA extraction from fungus was developed to expedite the technique. With this method, the usefulness of the Conio7 and Conio8 was verified using new Coniophora eremophila and An trodia sinuosa isolates from citrus. [References: 13]
机译：在西南沙漠中，棕色心材腐烂是一个日益重要的问题，影响了许多本地树种和商业柠檬生产。已鉴定出两种真菌病原体，即Coniophora eremophila和Antrodia sinuosa，是柑橘中棕色心材腐烂的病因。有效疾病管理策略的开发依赖于易于识别和区分病原体的能力。因此，我们已经开发出分子标记物作为形态学方法的替代方法，以区分这两种在经济上重要的病原体。随机扩增多态性DNA分析鉴定出四个揭示真菌多态性的引物。克隆，测序多态性DNA片段，并用于使用序列特异性引物Conio7和Conio8在嗜血气单胞菌分离物中创建一个700 bp的序列，用于表征扩增区（SCAR）。用Conio7和Conio8在中华曲霉分离物中扩增出三个片段（300、650和1000 bp）。为了加快该技术的发展，开发了一种快速，小规模的从真菌中提取DNA的方法。通过这种方法，使用新的Coniophora eremophila和柑橘中的trodia sinuosa分离物验证了Conio7和Conio8的有用性。 [参考：13]

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