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首页> 外文期刊>Mycologia >The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities
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The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities

机译：白腐菌侧耳平菇分泌具有不同底物特异性的漆酶同工酶

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摘要

Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized by SDS-PAGE, estimating their molecular masses around 80 and 82 kDa, respectively. By native isoelectrofocusing, their pI values were 4.7 and 4.5, respectively. When staining with ABTS and guaiacol in native polyacrilamide gels, different specificities were observed for LCC1/LCC2 and LCC3/LCC4 isozymes.
机译：纯化并鉴定了平菇平菇V-184合成的四种漆酶同工酶（LCC1，LCC2，LCC3和LCC4）。 LCC1和LCC2的分子量分别约为60和65 kDa，并具有相同的pI值（3.0）。他们的N末端被测序，揭示了相同的氨基酸序列，并与其他微生物的漆酶具有同源性。漆酶LCC3和LCC4用SDS-PAGE表征，分别估计其分子量约为80和82 kDa。通过自然等电聚焦，它们的pI值分别为4.7和4.5。在天然聚丙烯酰胺凝胶中用ABTS和愈创木酚染色时，对于LCC1 / LCC2和LCC3 / LCC4同工酶观察到不同的特异性。

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