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首页> 外文期刊>Mycologia >IMMUNOCYTOCHEMICAL COMPARISON OF PERIPHERAL VESICLES IN ZOOSPORES OF PHYTOPHTHORA AND PYTHIUM SPECIES
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IMMUNOCYTOCHEMICAL COMPARISON OF PERIPHERAL VESICLES IN ZOOSPORES OF PHYTOPHTHORA AND PYTHIUM SPECIES

机译：疫霉菌和腐霉菌属物种的囊壁中外周血管的免疫细胞化学比较

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摘要

An ultrastructural and immunocytochemical comparison has been made of vesicles in the peripheral cytoplasm of zoospores of Phytophthora cinnamomi and species of the related Oomycete genus, Pythium. Our results give evidence of three morphologically and immunologically distinct vesicle populations in Pythium aphanidermatum and Py. butleri. Large peripheral vesicles can be recognized by their size and morphology, and by labeling with monoclonal antibody, Cpa-2 raised against a P. cinnamomi antigen. They occur predominantly on the dorsal surface of the zoospores and are retained within the cell during encystment. After encystment, the large peripheral vesicles move away from the plasma membrane and become distributed throughout the cyst cytoplasm, in a manner similar to that observed in P. cinnamomi. There are also small vesicles in the Pythium zoospore cortex. Some are identified as ventral vesicles through their reaction with monoclonal antibody Vsv-1, raised against a P. cinnamomi antigen. The ventral vesicles are concentrated along the ridges of the groove on the ventral surface of the zoospores, and their contents are secreted during encystment. The secreted material remains localized on the cyst surface and marks the site of germ tube emergence, as it does in P. cinnamomi. Other small peripheral vesicles occur on the dorsal surface of the Pythium zoospores. These vesicles are not labeled by any of the three antibodies tested but disappear during encystment, suggesting that their contents are secreted. Our results give strong support to the idea that three types of peripheral vesicles are a common feature of zoospores throughout the Peronosporales, and that they have similar fates during encystment. [References: 29]
机译：已对肉桂疫霉菌的游动孢子和相关的卵菌属，腐霉属的种的外围细胞质中的囊泡进行了超微结构和免疫细胞化学比较。我们的结果提供了在Pythium aphanidermatum和Py中三个形态和免疫学上不同的囊泡种群的证据。巴特里。大的外周囊泡可以通过其大小和形态来识别，并且可以通过用单克隆抗体标记针对P. cinnamomi抗原的Cpa-2进行识别。它们主要发生在游动孢子的背面，并在包囊过程中保留在细胞内。包囊后，大的外周囊泡从质膜上移开，并分布在整个囊肿细胞质中，其方式类似于在肉桂假单胞菌中观察到的方式。腐霉菌的游动孢子皮层中也有小囊泡。一些通过与单克隆抗体Vsv-1的反应而被鉴定为腹小泡，该抗体针对P. cinnamomi抗原。腹小泡沿游动孢子的腹表面上的凹槽的脊部集中，并且其内容物在包囊过程中被分泌。就像在肉桂对虾中一样，分泌的物质仍保留在囊肿表面上并标记出胚芽管的出现位置。其他小的外围囊泡出现在腐霉菌孢子的背面。这些囊泡没有被测试的三种抗体中的任何一种标记，但在包囊过程中消失，表明它们的内容物被分泌出来。我们的结果为以下观点提供了有力的支持：三种类型的外围囊泡是整个Peronosporales中游动孢子的共同特征，并且它们在包囊过程中具有相似的命运。 [参考：29]

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