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Liquid culture and growth quantification of the seagrass pathogen, Labyrinthula spp

机译：海草病原体，迷宫菌的液体培养和生长定量

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摘要

Symptoms characteristic of wasting disease, thought to result from infection by protozoan pathogens (i.e. Labyrinthula spp.), are a common phenomenon affecting seagrass species worldwide. However relatively little is known about factors that control the survival and success of Labyrinthula in part due to the difficulty associated with quantifying the growth of this organism. Here we describe a simple and inexpensive method for measuring growth of Labyrinthula in liquid culture that takes into consideration both cell density and areal spread. The technique allows for examination of the effects of both abiotic and biotic factors on the growth of Labyrinthula apart from its seagrass host, separating the effects of environmental condition on the host from their effects on the pathogen.
机译：浪费性疾病的症状特征被认为是原生动物病原体（即迷宫菌）感染所致，是影响全世界海草种类的常见现象。然而，关于控制迷宫的生存和成功的因素知之甚少，部分是由于与定量该生物的生长有关的困难。在这里，我们描述了一种既简单又便宜的方法，用于测量液体培养中迷宫的生长，该方法考虑了细胞密度和面积分布。该技术允许检查非生物和生物因素对迷宫藻（除其海草宿主以外）生长的影响，从而将环境条件对宿主的影响与对病原体的影响分开。

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