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首页> 外文期刊>Mycologia >Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota
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Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota

机译：木炭滤纸可提高冷冻保存的丝状外生菌根和腐生的担子菌和子囊菌的活力

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摘要

We assessed viability of 18 strains of filamentous ectomycorrhizal and saprotrophic basidiomycetes and ascomycetes after cryopreservation with a novel technique based on charcoal filter paper strips (CFS). The results indicate that axenic fungal cultures grown on CFS recovered from freezing within a few days, even though none survived cryopreservation by the conventional straw method. Fungal growth on CFS was more vigorous, with morphological differentiations such as rhizomorphs and an increased amount of aerial mycelia compared to the unamended culture media. Accordingly CFS allows the cryopreservation of a wide range of rare and important ectomycorrhizal and saprotrophic fungi, which hitherto were difficult to revive from liquid nitrogen storage with the conventional and widely applied straw technique.
机译：我们用一种基于木炭滤纸条（CFS）的新技术，在冷冻保存后评估了18株丝状外生菌根和腐生的担子菌和子囊菌的活力。结果表明，在CFS上生长的轴突真菌培养物在几天之内即可从冰冻状态中恢复，即使没有通过常规秸秆冷冻保存的方法。与未经改良的培养基相比，CFS上的真菌生长更加旺盛，具有形态学差异，例如根状菌和气生菌丝数量增加。因此，CFS可以冷冻保存各种稀有和重要的外生菌根和腐生真菌，这些真菌迄今难以通过常规和广泛应用的秸秆技术从液氮储存中恢复出来。

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