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Serenomyces associated with palms in southeastern USA: isolation, culture storage and genetic variation

机译:美国东南部与棕榈树相关的拟皮动物:隔离,培养物贮藏和遗传变异

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摘要

Serenomyces is a genus belonging to the family Phaeochoraceae, which is known to occur only in association with the plant family Arecaceae (palms). It is presumed to be one of the causes of a leaf disease referred to as either rachis blight or petiole blight, depending on the palm species affected. The fungus is not readily observed, with few reports in the literature; it cannot be readily isolated from tissue, with only one known instance of it being cultured on artificial media and has no DNA sequences deposited in GenBank. Over an 8 y period, leaves symptomatic for rachis or petiole blight were obtained from Florida and South Carolina, USA. The fungus was induced to produce ascospores, and single-spore isolates were obtained in culture and, in some instances, induced to produce ascospores in culture. Based on ascospores size and ITS sequencing, Serenomyces from Phoenix canariensis and P. dactylifera form one group, Serenomyces from Thrinax radiata form a second group and Serenomyces from Sabal palmetto form a third. All three groups are most similar morphologically to Serenomyces phoenicis. Due to the observed instability of Serenomyces in culture, we have suggestions regarding the storage of this fungus
机译:塞氏菌属于Phaeochoraceae科,已知仅与植物Arecaceae(棕榈)有关。据推测,这是导致叶片疾病的原因之一,称为叶枯病或叶柄枯萎病,具体取决于受影响的棕榈树种。这种真菌不容易观察到,文献报道很少。它不能容易地从组织中分离出来,只有一个已知的例子是在人工培养基上培养的,并且GenBank中没有DNA序列。在8年内,从美国佛罗里达州和南卡罗来纳州获得有症状的叶片或叶柄枯萎病的叶片。诱导真菌产生子孢子,并在培养物中获得单孢子分离物,并且在某些情况下,诱导在培养物中产生子孢子。基于子囊孢子大小和ITS测序,Phoenix canariensis和P. dactylifera的致病菌组成第二组,Thinax radiata的致病菌形成第二组,Sabal palmetto的致病菌形成第三组。所有这三个组在形态上都与菲氏志贺菌相似。由于观察到的菌丝菌在文化中的不稳定性,我们对这种真菌的储存提出了一些建议。

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