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首页> 外文期刊>Mycologia >Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis
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Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis

机译：线粒体小孔亚单位核糖体DNA的扩增及其在系统发育分析中的潜力

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摘要

There has been a systematic need to seek adequate phylogenetic markers that can be applied in phylogenetic analyses of fungal taxa at various levels. The mitochondrial small subunit ribosomal DNA (mt SSU rDNA) is generally considered to be one of the molecules that are appropriate for phylogenetic analyses at a family level. In order to obtain universal primers for polypores of Hymenomycetes, mt SSU rRNA genes were cloned from Bjerkandera adusta, Ganoderma lucidum, Phlebiopsis gigantea, and Phellinus laevigatus and their sequences were determined. Based on the conserved sequences of cloned genes from polypores and Agrocybe aegerita, PCR primers were designed for amplification and sequencing of mt SSU rDNAs. New primers allowed effective amplification and sequencing of almost full-sized genes from representative species of polypores and related species. Phylogenetic relationships were resolved quite efficiently by mt SSU rDNA sequences, and they proved to be more useful in phylogenetic reconstruction of Ganoderma than nuclear internal transcribed spacer (ITS) rDNA sequences. [References: 33]
机译：已经系统地需要寻找可以在各种水平上用于真菌分类群的系统发育分析的适当的系统发育标记。线粒体小亚单位核糖体DNA（mt SSU rDNA）通常被认为是适合家庭水平系统发育分析的分子之一。为了获得血丝菌多孢子的通用引物，从Bjerkandera adusta，Ganoderma lucidum，Phlebiopsis gigantea和Phellinus laevigatus克隆了mt SSU rRNA基因，并确定了它们的序列。基于来自多孔和农杆菌的克隆基因的保守序列，设计PCR引物用于mt SSU rDNA的扩增和测序。新的引物可有效扩增和测序来自代表性多孔菌种和相关菌种的几乎全基因。系统发育关系可以通过mt SSU rDNA序列非常有效地解决，并且事实证明，它们比灵核内部转录间隔区（ITS）rDNA序列在灵芝的系统发育重建中更有用。 [参考：33]

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