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首页> 外文期刊>Mycologia >Fungal communities of young and mature hypersaline microbial mats.
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Fungal communities of young and mature hypersaline microbial mats.

机译：年轻和成熟的高盐微生物垫的真菌群落。

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Microbial mats are a laminated organic-sedimentary ecosystem, found in a wide range of habitats. Fluctuating diel and seasonal physicochemical gradients characterize these ecosystems, resulting in both strata and microenvironments that harbor specific microbial communities. This study was undertaken to compare two types of microbial mats across seasons to further understand the structure of fungal communities in hypersaline microbial mats and their seasonal dynamics. The structure and diversity of fungal communities was documented in young transient and mature hypersaline microbial mats from a tropical region (Puerto Rico) using one culture-dependent and three culture-independent molecular techniques based on the internal transcribed spacer (ITS) region of ribosomal DNA: terminal restriction fragment length polymorphism (TRFLP), denaturing gradient gel electrophoresis (DGGE) and clone libraries. Two microbial mats (one young and transient, one mature) were sampled in Nov 2007 (wet season), Jan 2008 (intermediate season) and Mar 2008 (dry season) in the Cabo Rojo Solar Salterns on the southwestern coast of Puerto Rico. Traditional and molecular techniques revealed strong spatial and temporal heterogeneities in both microbial mats. Higher abundance of isolates and phylotypes were observed during the wet season, and diversity decreased from the top (oxic) to the bottom (anoxic) layers in both seasons. Some of the species isolated belong to the genera Aspergillus, Cladosporium, Hortaea, Pichia and Wallemia, which often are isolated from hypersaline environments. The most abundant clones belong to Acremonium strictum and Cladosporium halotolerans, which were not isolated in pure culture. The differences observed using culture-based and molecular techniques demonstrates the need of combining methods to study the diversity of fungi in a given substrate.
机译：微生物垫是一种层状的有机沉积生态系统，存在于广泛的栖息地中。波动的diel和季节性理化梯度代表了这些生态系统的特征，从而形成了具有特定微生物群落的地层和微环境。本研究旨在比较不同季节的两种微生物垫，以进一步了解高盐微生物垫中的真菌群落结构及其季节性动态。使用一种依赖于培养物和三种不依赖于培养物的分子技术，基于核糖体DNA的内部转录间隔区（ITS），在热带地区（波多黎各）的年轻短暂和成熟的高盐微生物垫中记录了真菌群落的结构和多样性。 ：末端限制性片段长度多态性（TRFLP），变性梯度凝胶电泳（DGGE）和克隆文库。在波多黎各西南海岸的Cabo Rojo太阳盐沼中，分别于2007年11月（潮湿季节），2008年1月（中间季节）和2008年3月（干燥季节）采样了两种微生物垫（一种年轻且短暂，一种成熟）。传统和分子技术揭示了两种微生物垫在空间和时间上的异质性。在雨季期间，分离物和系统型的丰度更高，并且在两个季节中，从顶层（有氧）到底层（缺氧），多样性都降低了。分离出的一些物种属于曲霉属，枝孢属，霍塔亚，毕赤酵母和Wallemia属，它们通常与高盐环境分离。最丰富的克隆属于严格的顶头孢霉和卤化克氏孢子，它们不是在纯培养物中分离的。使用基于培养物的技术和分子技术观察到的差异表明，需要结合各种方法来研究给定底物中真菌的多样性。

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