...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >Germ cell transplantation from large domestic animals into mouse testes
【24h】

Germ cell transplantation from large domestic animals into mouse testes

机译：从大型家畜到小鼠睾丸的生殖细胞移植

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Donor-derived spermatogenesis after spermatogonial transplantation to recipient animals could serve as a novel approach to manipulate the male germ line in species where current methods of genetic modification are still inefficient. The objective of the present study was to investigate germ cell transplantation from boars, bulls, and stallions, which are economically important domestic animals, to mouse recipients. Donor testis cells (fresh, cryopreserved, or cultured for 1 month) were transplanted into testes of immunodeficient recipient mice in which endogenous spermatogenesis had been destroyed. Recipient testes were analyzed from 1 to > 12 months after transplantation for the presence of donor germ cells by donor-specific immunohistochemistry. Donor cells were present in most recipient testes with species-dependent differences in pattern and extent of colonization. Porcine donor germ cells formed chains and networks of round cells connected by intercellular bridges but later stages of donor-derived speumatogenesis were not observed. Transplanted bovine testis cells initially appeared similar but then developed predominantly into fibrous tissue within recipient seminiferous tubules. Few equine germ cells proliferated in mouse testes with no obvious difference between cells recovered from a scrotal or a cryptorchid donor testis. The pattern of colonization after transplantation of cultured cells did not resemble spermatogonial proliferation. These results indicate that fresh or cryopreserved germ cells from large animals can colonize the mouse testis but do not differentiate beyond the stage of spermatogonial expansion. Species-specific differences in the compatibility of large animal donors and mouse recipients were detected which cannot be predicted solely on the basis of phylogenetic distance between donor and recipient species. Mol. Reprod. Dev. 57:270-279, 2000.
机译：在目前的遗传修饰方法仍然无效的情况下，精原细胞移植到受体动物后，供体衍生的精子发生可以作为一种操纵雄性种系的新方法。本研究的目的是研究从经济上重要的家畜公猪，公牛和种马到小鼠接受者的生殖细胞移植。将供体睾丸细胞（新鲜，冷冻保存或培养1个月）移植到免疫缺陷受体小鼠的睾丸中，其中内源性精子发生已被破坏。移植后1至12个月，通过供体特异性免疫组织化学分析接受者的睾丸中供体生殖细胞的存在。供体细胞存在于大多数受体睾丸中，定植模式和程度的差异取决于物种。猪供体生殖细胞形成了通过细胞间桥连接的圆形细胞链和网络，但未观察到供体衍生的粉尘形成的后期阶段。移植的牛睾丸细胞最初看起来相似，但随后主要发育到受体生小管内的纤维组织中。在小鼠睾丸中增殖的马生殖细胞很少，从阴囊或隐睾供体睾丸中回收的细胞之间没有明显差异。培养细胞移植后的定植模式与精原细胞增殖不同。这些结果表明，来自大型动物的新鲜或冷冻保存的生殖细胞可以在小鼠睾丸中定植，但在精原细胞扩张阶段不会分化。检测到大型动物供体和小鼠受体相容性的物种特异性差异，这不能仅根据供体和受体物种之间的系统发育距离来预测。大声笑责备。开发人员57：270-279，2000。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号