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首页> 外文期刊>Molecular reproduction and development >Prostasomal DNA Characterization and Transfer Into Human Sperm
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Prostasomal DNA Characterization and Transfer Into Human Sperm

机译：前体DNA表征和转移到人类精子

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Human prostasomes, exosome-like microvesicles secreted by acinar cells of the prostate gland, contain chromosomal DNA. Agarose gel electrophoresis of DNA from seminal prostasomes displayed fragments of over 12 kb and smaller, with a distinct band around 1 kb that was excised, cloned, and sequenced. The sequences showed 8 out of 25 clones (32%) originating from genes. We elaborated the concept further by carrying out a genome-wide DNA copy number analysis of prostasomal DNA, hypothesizing that human prostasomes contain fragments of DNA randomly selected from the entire genome. Acridine orange-stained prostasomes were incubated with freshly prepared sperm for different times, and a transfer of acridine orange-stained prostasomal DNA to sperm (preferentially the head region) was observed. Fluorescence microscopy of slices in the center of 14 optical slides of the sperm head displayed an even fluorescence rather than a halo-like one, indicating DNA-uptake rather than just binding along the sperm head membrane. Mol. Reprod. Dev. 78: 467-476, 2011
机译：人前列腺，由前列腺腺泡细胞分泌的外泌体样微囊泡，含有染色体DNA。来自精子前体的DNA的琼脂糖凝胶电泳显示了超过12 kb和更小的片段，在1 kb左右有明显的条带被切除，克隆和测序。序列显示出25个克隆中的8个（32％）源自基因。我们通过对前体DNA进行全基因组DNA拷贝数分析来进一步阐述该概念，并假设人类前体包含从整个基因组中随机选择的DNA片段。将fresh啶橙染色的前体与新鲜制备的精子孵育不同的时间，并观察到of啶橙染色的前体DNA向精子（最好是头部）的转移。精子头的14个光学载玻片中心的切片的荧光显微镜显示均匀的荧光，而不是类似晕轮的荧光，这表明DNA的摄取而不仅仅是沿着精子头膜的结合。大声笑责备。开发人员78：467-476，2011年

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