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首页> 外文期刊>Mycologia >Long-term survival of Glomus claroideum propagules from soil pot culturesunder simulated conditions
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Long-term survival of Glomus claroideum propagules from soil pot culturesunder simulated conditions

机译：模拟条件下土壤盆栽培养的球状云母繁殖体的长期存活

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This study was initiated to determine the effect of storage conditions on Glomus claroideum. Pure cultures of this AM fungus containing approximately 80 spores/g were stored in Dothan soil under four temperature-moisture regimens: room temperature 24 C at field capacity (FC) and at 50% FC and refrigeration (4 C) at FC and at 50% FC. Soil samples were taken at 0, 2, and 4 wk, monthly thereafter until 48 wk, and then at 72, 104, 156 and 272 wk and assessed for spore numbers by sucrose gradient centrifugation. Spore viability was also assessed by most probable number and by vital staining with 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT). Spore losses were less when cultures were stored at 4 C than at 24 C. The half-life of G. claroideum spores was approximately 2 yr at 24 C and 3.5 yr at 4 C. Spore numbers of G. claroideum stored for 272 wk at 24 C and both moisture levels decreased from 80 to 12 spores/g while those stored at 4 C decreased to approximately 30 spores/g. Most probable numbers of total G. claroideum propagules stored at 4 C were significantly higher than cultures stored at 24 C. Temperature was more critical than soil moisture for survival. Even after 272 wk at 4 C, there were sufficient viable propagules of G. claroideum (300/g) to be used as an inoculum for studies with AM fungi. Viability staining demonstrated that the percentage of viable spores decreased with time of storage. These results demonstrate that the best method of AM fungi culture storage in Dothan soil is at 4 C and moisture conditions less than or equal to field capacity.
机译：这项研究的开始是为了确定贮藏条件对金球藻的影响。将这种含有大约80孢子/克的AM真菌的纯培养物在以下四种温度-湿度方案下储存在Dothan土壤中：室温下24 C，田间持水量（FC）和50％FC，冷藏（4 C），FC和50 ％FC。在0、2和4 wk采集土壤样品，此后每月一次，直到48 wk，然后在72、104、156和272 wk采集，并通过蔗糖梯度离心法评估孢子数。还通过最可能的数量和2-（对碘苯基）-3-（对硝基苯基）-5-苯基-2H-四唑鎓氯化物（INT）的活体染色来评估孢子生存力。当将培养物在4 C储存时，孢子损失比在24 C时少。在24 C时，锁齿灵芝孢子的半衰期约为2年，在4 C时，其半衰期为3.5年。在25°C贮存272 wk的锁齿灵芝的孢子数24 C，两种水分含量均从80孢子/ g降低至4 C时，降低至约30孢子/ g。在4 C下储存的最总G. claroideum繁殖体的数量明显高于在24 C下储存的培养物。温度比土壤湿度对生存至关重要。即使在4 C下272 wk之后，仍然有足够的繁殖的G. claroideum（300 / g）繁殖体用作AM真菌研究的接种物。生存力染色表明，存活孢子的百分比随储存时间的延长而降低。这些结果表明，在Dothan土壤中AM真菌培养物贮藏的最佳方法是在4 C和湿度条件下小于或等于田间持水量。

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