...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mycologia >ESTIMATING THE VIABILITY OF VESICULAR-ARBUSCULAR MYCORRHIZAE FUNGAL SPORES USING TETRAZOLIUM SALTS AS VITAL STAINS
【24h】

ESTIMATING THE VIABILITY OF VESICULAR-ARBUSCULAR MYCORRHIZAE FUNGAL SPORES USING TETRAZOLIUM SALTS AS VITAL STAINS

机译：使用四唑盐作为活菌估计猪肺泡真菌支原体的可行性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The tetrazolium salts 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide and 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride were evaluated as viral stains for estimating the viability of fungal spores of Glomus mosseae and Glomus clarum. Respiring fungal spores accumulated tetrazolium bromide-formazan and tetrazolium chloride-formazan in the cytoplasm as a visually distinct color reaction. Estimates, however, of fungal spore viability based on tetrazolium bromide staining were inconsistent with viability estimates based on a bioassay using single spores of G. clarum. Inconsistencies were due largely to the ambiguous interpretation of the significance of black spores. In contrast, color reactions in tetrazolium chloride were relatively stable and consistent with bioassay estimates of viability. Generally, incubation in 1 mg ml(-1) tetrazolium chloride for 48 to 72 h was sufficient to ensure maximum color reaction. Viable spores were distinguishable from those which were unable to colonize a host plant on the basis of the intensity of color reaction in tetrazolium chloride. We conclude that tetrazolium chloride staining can be used as a rapid indicator of vesicular-arbuscular mycorrhizae fungal spore viability. [References: 24]
机译：四唑盐3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四唑鎓和2-（对碘苯基）-3-（对硝基苯基）-5-苯基-2H-将氯化四唑鎓作为病毒染色剂进行评估，以评估Glomus mosseae和Glomus clarum真菌孢子的活力。呼吸性真菌孢子在细胞质中积累了溴化四氮唑-甲a和氯化四氮唑-甲a，这是一种视觉上明显的颜色反应。然而，基于溴化四氮唑染色的真菌孢子生存力估计与基于使用克拉克单孢子的生物测定法进行的生物测定的生存能力估计不一致。不一致主要是由于对黑孢子的重要性的模棱两可的解释。相比之下，氯化四唑鎓中的显色反应相对稳定，并且与生物测定法中的生存力估计值一致。通常，在1 mg ml（-1）氯化四氮唑中孵育48至72小时足以确保最大程度的显色反应。根据氯化四氮唑中显色反应的强度，可以将活孢子与不能在寄主植物中定殖的孢子区分开。我们得出结论，氯化四唑鎓染色可以用作水泡-丛枝菌根真菌孢子活力的快速指示剂。 [参考：24]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号