Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola.

Anderson DW; Nicholson RL

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Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola.

机译：炭疽菌分生孢子粘液中漆酶的特征。

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A polyphenol oxidase was demonstrated to be a component of the extracellular conidial mucilage of C. graminicola. The enzyme was identified as a laccase based on its oxidation of syringaldazine in the absence of hydrogen peroxide and its absorptionmax. at 593 nm, that is characteristic of blue, type II copper-containing laccases. The enzyme, purified by DEAE cellulose, Superose and MONO Q Fast Protein Liquid chromatographic procedures, had a MW of 85 kDa, an opt. pH 6.0 and exhibited Km and Vmax values of 0.214 mmoles and 1.014 mmoles/litre/min, respectively, with syringaldazine as the substrate. The function of the enzyme is discussed relative to its potential role in fungal survival and detoxification of plant phenols.

机译：已证明多酚氧化酶是粒状梭菌胞外分生孢子黏液的成分。基于在没有过氧化氢的情况下丁香醛嗪的氧化和最大吸收，该酶被鉴定为漆酶。在593 nm处具有蓝色II型含铜漆酶的特征。通过DEAE纤维素，Superose和MONO Q快速蛋白质液相色谱程序纯化的酶，分子量为85 kDa（可选）。 pH为6.0，以丁香达拉嗪为底物，其Km和Vmax值分别为0.214 mmoles和1.014 mmoles / L / min。讨论了酶的功能及其在真菌存活和植物酚解毒中的潜在作用。

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《Mycologia》 |1996年第6期|共7页
作者
Anderson DW; Nicholson RL;
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原文格式 PDF
正文语种 eng
中图分类 Q939.5;
关键词
plant pathogens; plant pathogenic fungi; biochemistry enzymes plant pathology; Colletotrichum graminicola;

机译：植物病原体;植物病原真菌;生化酶;植物病理学;Graetotrichum graminicola;

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专利

1. Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola. [J] . Anderson DW, Nicholson RL Mycologia . 1996,第6期

机译：炭疽菌分生孢子粘液中漆酶的特征。
2. LAC2 Encoding a Secreted Laccase Is Involved in Appressorial Melanization and Conidial Pigmentation in Colletotrichum orbiculare [J] . Shao Yu Lin, Shiho Okuda, Kyoko Ikeda, Molecular Plant-Microbe Interactions . 2012,第12期

机译：编码分泌的漆酶的LAC2参与炭疽菌的阑尾变黑和分生色素沉着
3. Partial characterization of the extracellular matrix released onto hydrophobic surfaces by conidia and conidial germlings of Colletotrichum graminicola [J] . Sugui JA, Leite B, Nicholson RL Physiological and Molecular Plant Pathology . 1998,第6期

机译：炭疽菌的分生孢子和分生孢子幼苗释放到疏水表面的细胞外基质的部分表征
4. Preparation and Characterization of Okra Mucilage (Abelmoschus esculentus) Edible Films [C] . Monique de Alvarenga Pinto Cotrim, Artur Caron Mottin, Eliane Ayres Brazilian Polymer Conference . 2016

机译：秋葵（Abelmoschus Esculentus）可食用薄膜的制备与表征
5. A class V chitin synthase, chsA, and its roles in cell wall integrity in the fungus Colletotrichum graminicola. [D] . Amnuaykanjanasin, Alongkorn. 2003

机译：V类几丁质合酶chsA及其在真菌Colletotrichum graminicola中的细胞壁完整性中的作用。
6. Purification and characterization of the conidial laccase of Aspergillus nidulans. [O] . M B Kurtz, S P Champe 1982

机译：构巢曲霉的分生孢子漆酶的纯化和鉴定。
7. LAC2 Encoding a Secreted Laccase Is Involved in Appressorial Melanization and Conidial Pigmentation in Colletotrichum orbiculare [O] . Shao Yu Lin, Shiho Okuda, Kyoko Ikeda, 2012

机译：编码分泌漆酶的LAC2涉及Colletottrichum orbiculare中的Meppressial Melanization和Concirial色素沉着

Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola.

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