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首页> 外文期刊>Mycologia >Characterization and phylogenetic analysis of the mating-type loci in the asexual ascomycete genus Ulocladium
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Characterization and phylogenetic analysis of the mating-type loci in the asexual ascomycete genus Ulocladium

机译：无性子囊虫Ulocladium交配型基因座的表征和系统发育分析

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摘要

The genus Ulocladium is thought to be strictly asexual. Mating-type (MAT) loci regulate sexual reproduction in fungi and their study may help to explain the apparent lack of sexual reproduction in Ulocladium. We sequenced the full length of two MAT genes in 26 Ulocladium species and characterized the entire MAT idiomorphs plus flanking regions of Ulocladium botrytis. The MAT1-1 ORF encodes a protein with an alpha-box motif by the MAT1-1-1 gene and the MAT1-2 ORF encodes a protein with an HMG box motif by the MAT1-2-1 gene. Both MAT1-1-1 and MAT1-2-1 genes were detected in a single strain of every species. Moreover, the results of RT-PCR revealed that both MAT genes are expressed in all 26 Ulocladium species. This demonstrates that MAT genes of Ulocladium species might be functional and that they have the potential for sexual reproduction. Phylogenies based on MAT genes were compared with GAPDH and Alt a 1 phylograms in Ulocladium using maximum parsimony (MP) and Bayesian analysis. The MAT genealogies and the non-MAT trees displayed different topologies, indicating that MAT genes are unsuitable phylogenetic markers at the species level in Ulocladium. Furthermore, the conflicting topologies between MAT1-1-1 and MAT1-2-1 phylogeny indicate separate evolutionary events for the two MAT genes. However, the intergeneric phylogeny of four closely allied genera (Ulocladium, Alternaria, Cochliobolus, Stemphylium) based on MAT alignments demonstrated that MAT genes are suitable for phylogenetic analysis among allied genera
机译：Ulocladium属被认为是严格无性的。交配型（MAT）基因座可调节真菌的性繁殖，他们的研究可能有助于解释Ulocladium中明显缺乏性繁殖的现象。我们对26种Ulocladium物种中两个MAT基因的全长进行了测序，并表征了整个MAT独特型以及Ulocladium botrytis的侧翼区域。 MAT1-1 ORF通过MAT1-1-1基因编码具有alpha框基序的蛋白质，MAT1-2 ORF通过MAT1-2-1基因编码具有HMG框基序的蛋白质。在每个物种的单个菌株中都检测到MAT1-1-1和MAT1-2-1基因。而且，RT-PCR的结果表明，两种MAT基因均在全部26种Ulocladium物种中表达。这表明Ulocladium物种的MAT基因可能起作用，并且具有性繁殖的潜力。使用最大简约（MP）和贝叶斯分析，将基于MAT基因的系统发育与Ulocladium中的GAPDH和Alt a 1系统进化图进行了比较。 MAT族谱和非MAT树显示出不同的拓扑结构，表明MAT基因在Ulocladium中在物种水平上是不合适的系统发育标记。此外，MAT1-1-1和MAT1-2-1系统发育之间相互冲突的拓扑表明两个MAT基因的单独进化事件。然而，基于MAT比对的四个紧密相关属（Ulocladium，Alternaria，Cochliobolus，Stemphylium）的属间系统发育证明，MAT基因适合于相关属之间的系统发育分析。

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