...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Eosin, a potent inhibitor of the plasma membrane Ca pump, does not inhibit the cardiac Na-Ca exchanger.
【24h】

Eosin, a potent inhibitor of the plasma membrane Ca pump, does not inhibit the cardiac Na-Ca exchanger.

机译：曙红是质膜Ca泵的有效抑制剂，不会抑制心脏Na-Ca交换剂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Na-Ca exchanger and the sarcolemmal/plasma membrane (SL(PM)) Ca pump are the two major pathways for Ca transport to the extracellular space in many cells. In cardiac myocytes, the Na-Ca exchanger appears to be responsible for a greater portion of this Ca flux [Bassani, R. A., et al. (1992) J. Physiol. 453, 591-608]. However, the respective contributions of these two transporters are not as well-defined in all tissues (e.g., smooth muscle). We propose that eosin (tetrabromofluorescein) may be a useful tool for quantitatively determining the proportion of Ca transported by the Na-Ca exchanger vs the SL(PM) Ca pump in various cells. Eosin is the most potent inhibitor known for the SL(PM) Ca pump (IC50 approximately 0.3 microM in red blood cell inside-out vesicles); unlike the Na/K and H/K pumps, eosin does not compete with ATP for the SL(PM) Ca pump [Gatto, C., & Milanick, M. A. (1993) Am. J. Physiol. 264, C1577-C1586]. In the present study, we have shown that eosin was a potent inhibitor of the cardiac SL(PM) Ca pump (IC50 approximately 1 microM); in contrast, eosin (< or = 20 microM) did not inhibit the cardiac Na-Ca exchanger. In experiments where Ca was being transported by both the SL(PM) Ca pump and the Na-Ca exchanger simultaneously, eosin effectively eliminated the Ca pump-mediated transport. In addition, we show that eosin can permeate the human red cell membrane; cell permeability is an attractive feature for using eosin in whole cell studies. We conclude that eosin can be used for determining the role that the SL(PM) Ca pump plays in whole cell Ca homeostasis.
机译：Na-Ca交换子和肌膜/质膜（SL（PM））Ca泵是Ca转运到许多细胞中细胞外空间的两个主要途径。在心肌细胞中，Na-Ca交换子似乎是造成Ca流量更大的原因[Bassani，R. A.，et al。 （1992）J.Physiol。 453，591-608]。然而，在所有组织（例如，平滑肌）中，这两种转运蛋白各自的作用尚未明确。我们建议曙红（四溴荧光素）可能是一种有用的工具，用于定量确定Na-Ca交换器与SL（PM）Ca泵在各种细胞中运输的Ca的比例。曙红是SL（PM）Ca泵已知的最有效抑制剂（红细胞内外囊泡中IC50约为0.3 microM）;与Na / K和H / K泵不同，曙红不能与ATP竞争SL（PM）Ca泵[Gatto，C.，＆Milanick，M. A.（1993）Am。 J.生理学。 264，C1577-C1586]。在本研究中，我们显示曙红是心脏SL（PM）Ca泵的有效抑制剂（IC50约为1 microM）。相反，曙红（<或= 20 microM）没有抑制心脏Na-Ca交换剂。在通过SL（PM）Ca泵和Na-Ca交换器同时运输Ca的实验中，曙红有效地消除了Ca泵介导的运输。此外，我们显示曙红可以渗透人类红细胞膜;细胞渗透性是在整个细胞研究中使用曙红的诱人特征。我们得出结论，曙红可用于确定SL（PM）Ca泵在全细胞Ca稳态中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号