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首页> 外文期刊>Mycologia >Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes
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Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes

机译：多孔菌中木质素降解的过氧化物酶：基于10个测序基因组的进化调查

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The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) were sequenced to expand our knowledge on the diversity of ligninolytic and related peroxidase genes in this Basidiomycota order that includes most wood-rotting fungi. The survey was completed by analyzing the heme-peroxidase genes in the already available genomes of seven more Polyporales species representing the antrodia, gelatoporia, core polyporoid and phlebioid clades. The study confirms the absence of ligninolytic peroxidase genes from the manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP) families, in the brown-rot fungal genomes (all of them from the antrodia clade), which include only a limited number of predicted low redox-potential generic peroxidase (GP) genes. When members of the heme-thiolate peroxidase (HTP) and dye-decolorizing peroxidase (DyP) superfamilies (up to a total of 64 genes) also are considered, the newly sequenced B. adusta appears as the Polyporales species with the highest number of peroxidase genes due to the high expansion of both the ligninolytic peroxidase and DyP (super)families. The evolutionary relationships of the 111 genes for class-II peroxidases (from the GP, MnP, VP, LiP families) in the 10 Polyporales genomes is discussed including the existence of different MnP subfamilies and of a large and homogeneous LiP cluster, while different VPs mainly cluster with short MnPs. Finally, ancestral state reconstructions showed that a putative MnP gene, derived from a primitive GP that incorporated the Mn (ID-oxidation site, is the precursor of all the class-II ligninolydc peroxidases. Incorporation of an exposed tryptophan residue involved in oxidative degradation of lignin in a short MnP apparently resulted in evolution of the first VP. One of these ancient VPs might have lost the Mn (II)oxidation site being at the origin of all the LiP enzymes, which are found only in species of the order Polyporales.
机译：对三个有代表性的多孔菌（Bjerkandera adusta，Phlebia brevispora和灵芝复合体的一个成员）的基因组进行了测序，以扩展我们对木质素分解和相关过氧化物酶基因多样性的了解，这一基础包括了大多数木腐真菌。这项调查是通过分析另外七种多孢菌物种的血红素过氧化物酶基因而完成的，这些基因已经代表了牛樟芝，明胶孢子，多孢子核心和类静脉进化枝。这项研究证实了褐腐真菌基因组（所有这些都来自牛樟芝）中的锰过氧化物酶（MnP），木质素过氧化物酶（LiP）和通用过氧化物酶（VP）家族都没有木质素分解过氧化物酶基因。数量有限的预测的低氧化还原电位通用过氧化物酶（GP）基因。当还考虑血红素-硫醇盐过氧化物酶（HTP）和染料脱色过氧化物酶（DyP）超家族的成员（最多64个基因）时，新测序的A.stas B. adusta似乎是过氧化物酶数量最多的多孢菌属。由于木质素分解过氧化物酶和DyP（超级）家族的高度扩展，这些基因的存在。讨论了10个多孔基因组中II类过氧化物酶（来自GP，MnP，VP，LiP家族）的111个基因的进化关系，包括存在不同的MnP亚家族和大型且均质的LiP簇，而不同的VP主要是短MnPs簇。最后，祖先状态重建表明，推定的MnP基因是从原始GP掺入了Mn（ID氧化位点）后形成的，是所有II类木质素过氧化物酶的前体。短MnP中的木质素显然导致了第一个VP的进化，其中一个古老的VP可能失去了所有LiP酶起源的Mn（II）氧化位点，而这些LiP酶仅在多孔目物种中才发现。

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