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首页> 外文期刊>Mycologia >Genetic diversity of Kenyan native oyster mushroom (Pleurotus)
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Genetic diversity of Kenyan native oyster mushroom (Pleurotus)

机译：肯尼亚本土牡蛎蘑菇（侧耳）的遗传多样性

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Members of the genus Pleurotus, also commonly known as oyster mushroom, are well known for their socioeconomic and biotechnological potentials. Despite being one of the most important edible fungi, the scarce information about the genetic diversity of the species in natural populations has limited their sustainable utilization. A total of 71 isolates of Pleurotus species were collected from three natural populations: 25 isolates were obtained from Kakamega forest, 34 isolates from Arabuko Sokoke forest and 12 isolates from Mount Kenya forest. Amplified fragment length polymorphism (AFLP) was applied to thirteen isolates of locally grown Pleurotus species obtained from laboratory samples using five primer pair combinations. AFLP markers and internal transcribed spacer (ITS) sequences of the ribosomal DNA were used to estimate the genetic diversity and evaluate phylogenetic relationships, respectively, among and within populations. The five primer pair combinations generated 293 polymorphic loci across the 84 isolates. The mean genetic diversity among the populations was 0.25 with the population from Arabuko Sokoke having higher (0.27) diversity estimates compared to Mount Kenya population (0.24). Diversity between the isolates from the natural population (0.25) and commercial cultivars (0.24) did not differ significantly. However, diversity was greater within (89%; P > 0.001) populations than among populations. Homology search analysis against the GenBank database using 16 rDNA ITS sequences randomly selected from the two clades of AFLP dendrogram revealed three mushroom species: P. djamor, P. floridanus and P. sapidus; the three mushrooms form part of the diversity of Pleurotus species in Kenya. The broad diversity within the Kenyan Pleurotus species suggests the possibility of obtaining native strains suitable for commercial cultivation.
机译：平菇属的成员，通常也称为牡蛎蘑菇，因其社会经济和生物技术潜力而闻名。尽管是最重要的食用真菌之一，但有关自然种群中物种遗传多样性的稀缺信息限制了其可持续利用。从三个自然种群中总共收集了71种平菇菌种：从卡卡梅加森林中获得25种分离物，从阿拉伯木树森林中分离出34种分离物，从肯尼亚山森林中分离出12种分离物。使用五个引物对组合，将扩增的片段长度多态性（AFLP）应用于从实验室样品中获得的13种本地生长的侧耳属菌种。核糖体DNA的AFLP标记和内部转录间隔区（ITS）序列分别用于估计种群之间和种群内部的遗传多样性并评估系统发生关系。五个引物对组合在84个分离物中产生了293个多态位点。种群之间的平均遗传多样性为0.25，而来自阿拉比科索科克（Arabuko Sokoke）的种群具有比（0.24）更高的肯尼亚估计多样性（0.27）。来自自然种群（0.25）和商业品种（0.24）的分离株之间的多样性没有显着差异。但是，（89％; P> 0.001）人群中的多样性比人群中的更大。利用从AFLP树状图的两个进化枝中随机选择的16个rDNA ITS序列，对GenBank数据库进行同源性搜索分析，发现了三种蘑菇种：P。djamor，P。floridanus和P. sapidus。这三种蘑菇是肯尼亚侧耳属物种多样性的一部分。肯尼亚侧耳属物种的广泛多样性表明，有可能获得适合商业化种植的天然菌株。

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