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首页> 外文期刊>Molecular reproduction and development >Organization of a gene coding for an oviduct-specific glycoprotein(oviductin) in the hamster
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Organization of a gene coding for an oviduct-specific glycoprotein(oviductin) in the hamster

机译：仓鼠输卵管特异性糖蛋白（oviductin）编码基因的组织

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摘要

Hamster oviductin, a high molecular weight glycoprotein secreted by the oviducts, is believed to participate in fertilization and protection of the tubal epithelium. Expression of the oviductin gene is confined strictly to nonciliated secretory cells of the oviduct and is regulated by hormones. The objective of this study was to characterize the genomic organization and to identify potential regulatory elements implicated in the control of transcription of the oviductin gene. Polymerase chain reaction was performed on hamster genomic DNA, yielding 2.2 kb of the 5' flanking region as well as 13.6 kb of genomic sequence comprising the entire coding sequence of the oviductin gene distributed in 11 exons. Sequencing of the 5' flanking region revealed, among other elements, an almost perfect estrogen-responsive element (GGTCACTGTGAC (T) under bar), an atypical TATA box (TAT (T) under bar AA), and a perfect inverted Spl site located between the transcription start site and the atypical TATA box. Primer extension analyses indicated that the hamster oviductin transcript possesses an unusually short 5' untranslated region of only 14 nucleotides. The distinct organization of the hamster oviductin gene in the vicinity of the transcription start site provides an interesting ground for further functional studies. Mel. Reprod. Dev. 57:238-246, 2000.
机译：仓鼠输卵管蛋白是输卵管分泌的一种高分子量糖蛋白，据信参与输卵管上皮的受精和保护。输卵管蛋白基因的表达严格限于输卵管的非纤毛分泌细胞，并受激素调节。这项研究的目的是表征基因组组织，并确定与输卵管蛋白基因的转录控制有关的潜在调控元件。在仓鼠基因组DNA上进行聚合酶链反应，产生2.2 kb的5'侧翼区域以及13.6 kb的基因组序列，其中包括分布在11个外显子上的输卵管蛋白基因的整个编码序列。对5'侧翼区域的测序显示，除其他因素外，几乎是一个完美的雌激素反应元件（bar下为GGTCACTGTGAC（T）），一个非典型的TATA盒（bar为AA下的TAT（T））和一个完美的倒Spl位点在转录起始位点和非典型TATA框之间。引物延伸分析表明，仓鼠输卵管转录物具有仅14个核苷酸的异常短的5'非翻译区。仓鼠输卵管蛋白基因在转录起始位点附近的独特组织为进一步的功能研究提供了有趣的基础。梅尔责备。开发人员57：238-246，2000。

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