...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >Active caspase-3 and ultrastructural evidence of apoptosis in spontaneous and induced cell death in bovine in vitro produced pre-implantation embryos.
【24h】

Active caspase-3 and ultrastructural evidence of apoptosis in spontaneous and induced cell death in bovine in vitro produced pre-implantation embryos.

机译：活性caspase-3和超微结构证据表明，牛在体外产生了植入前胚胎的自发性和诱导性细胞死亡。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study we investigated chronological onset and involvement of active caspase-3, apoptotic nuclear morphology, and TUNEL-labeling, as well as ultrastructural evidence of apoptosis, in both spontaneous and induced cell death during pre-implantation development of bovine in vitro produced embryos. Pre-implantation embryos (2-cell to Day 8 blastocysts) were cultured with either no supplementation (untreated) or with 10 microM staurosporine for 24 hr (treated). Embryos were subjected to immunohistochemical staining of active caspase-3, TUNEL-reaction for detection of DNA degradation and DAPI staining for detection of apoptotic nuclear morphology, and subjected to fluorescence microscopy. Additionally, treated and untreated blastocysts were fixed and processed for ultrastructural identification of apoptosis. Untreated embryos revealed no apoptotic features at 2- and 4-cell stages. However, active caspase-3 and apoptotic nuclear morphology were observed in an untreated 8-cell stage, and TUNEL-labeling was observed from the 16-cell stage. Blastomeres concurrently displaying all apoptotic features were present in a few embryos at 16-cell and morula stages and in all blastocysts. All three features were observed from the 8-cell stage in treated embryos, and blastomeres with apoptotic features appeared more numerous in treated than in untreated embryos. Ultrastructural evidence of apoptosis occurred with a comparable distribution pattern as apoptotic features detected by fluorescence microscopy in both treated and untreated blastocysts. Activation of caspase-3 is likely involved in both spontaneous and induced apoptosis in bovine pre-implantation embryos, and immunohistochemical staining of active caspase-3 may be used in combination with other markers to identify apoptosis in pre-implantation embryos.
机译：在这项研究中，我们调查了牛体外培养胚胎着床前自发和诱导的细胞死亡中活性caspase-3的发生时间和参与，凋亡核形态和TUNEL标记以及凋亡的超微结构证据。 。植入前的胚胎（2细胞至第8天的胚泡）在不添加（未处理）或10 microM星形孢菌素的情况下培养24小时（处理）。胚胎进行了活性caspase-3的免疫组织化学染色，TUNEL反应用于检测DNA降解，DAPI染色用于检测凋亡的核形态，并进行了荧光显微镜检查。另外，将处理过的和未处理过的胚泡固定并处理以用于凋亡的超微结构鉴定。未经处理的胚胎在2和4细胞阶段均未显示凋亡特征。但是，在未经处理的8细胞阶段观察到了活跃的caspase-3和凋亡核形态，从16细胞阶段观察到了TUNEL标记。同时显示所有凋亡特征的卵裂球存在于16个细胞和桑壳阶段的几个胚胎以及所有胚泡中。在处理过的胚胎中从8细胞阶段观察到所有这三个特征，并且具有凋亡特征的卵裂球在处理过的胚中比未处理的胚中出现更多。凋亡的超微结构证据与荧光显微镜在治疗和未治疗的囊胚中检测到的凋亡特征具有可比的分布模式。 caspase-3的激活可能参与了牛植入前胚胎的自发和诱导的凋亡，并且活性caspase-3的免疫组织化学染色可与其他标记物结合使用，以鉴定植入前胚胎的凋亡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号