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首页> 外文期刊>Molecular reproduction and development >Effect of Human Adipose Tissue-Derived Mesenchymal-Stem-Cell Bioactive Materials on Porcine Embryo Development
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Effect of Human Adipose Tissue-Derived Mesenchymal-Stem-Cell Bioactive Materials on Porcine Embryo Development

机译：人脂肪组织间充质干细胞生物活性物质对猪胚胎发育的影响

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Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) secrete bioac-tive materials that are beneficial for tissue repair and regeneration. In this study, we characterized human hAT-MSC bioactive material (hAT-MSC-BM), and examined the effectof hAT-MSC-BM on porcine embryo development. hAT-MSC-BM was enriched with several growth factors and cytokines, including fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA), and interleukin 6 (IL6). Among the various concentrations and days of treatment tested, 10% hAT-MSC-BM treatment beginning on culture Day 4 provided the best environment for the in vitro growth of parthenogenetic porcine embryos. While the addition of 10% fetal bovine serum (FBS) increased the hatching rate and the total cell number of parthenogenetic porcine embryos compared with the control and hAT-MSC culture medium group, the best results were from the group cultured with 10% hAT-MSC-BM. Mitochondrial activity was also higher in the 10% hAT-MSC-BM-treated group. Moreover, the relative mRNA expression levels of development and anti-apoptosis genes were significantly higher in the 10% hAT-MSC-BM-treated group than in control, hAT-MSC culture medium, or 10% FBS groups, whereas the transcript abundanceof an apoptosis gene was slightly lower. Treatment with 10% hAT-MSC-BM starting on Day 4 also improved the development rate and the total cell number of in vitro-fertilized embryos. This is the first report on the benefits of hAT-MSC-BM in a porcine embryo in vitro culture system. We conclude that hAT-MSC-BM is a new, alternative supplement that can improve the development of porcine embryos during both parthenogenesis and fertilization in vitro.
机译：人脂肪组织来源的间充质干细胞（hAT-MSC）分泌对组织修复和再生有益的生物活性物质。在这项研究中，我们表征了人类hAT-MSC生物活性物质（hAT-MSC-BM），并研究了hAT-MSC-BM对猪胚胎发育的影响。 hAT-MSC-BM富含多种生长因子和细胞因子，包括成纤维细胞生长因子2（FGF2），血管内皮生长因子A（VEGFA）和白介素6（IL6）。在测试的各种浓度和处理天数中，从培养第4天开始的10％hAT-MSC-BM处理为单性生殖猪胚胎的体外生长提供了最佳环境。与对照组和hAT-MSC培养基组相比，添加10％胎牛血清（FBS）可以提高孵化率和孤雌生殖猪胚胎的总细胞数，但最佳结果来自于用10％hAT- MSC-BM。在10％hAT-MSC-BM治疗组中，线粒体活性也更高。此外，在10％hAT-MSC-BM处理组中，发育和抗凋亡基因的相对mRNA表达水平显着高于对照组，hAT-MSC培养基或10％FBS组，而转录组的转录本丰富。凋亡基因略低。从第4天开始用10％hAT-MSC-BM处理也提高了发育速度和体外受精胚胎的总细胞数。这是关于hAT-MSC-BM在猪胚胎体外培养系统中的益处的首次报道。我们得出的结论是，hAT-MSC-BM是一种新的替代性补充品，可以在孤雌生殖和体外受精过程中改善猪胚胎的发育。

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