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首页> 外文期刊>Molecular reproduction and development >MMP-2 expression precedes the final ripening process of the bovine cervix.
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MMP-2 expression precedes the final ripening process of the bovine cervix.

机译：MMP-2表达在牛子宫颈的最终成熟过程之前。

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Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ripening process, serial cervical biopsies were obtained from 10 cows at Days 185 and 275 of pregnancy (approximately 5 days before calving), at parturition and at 30 days after parturition. The mRNA and protein expression of MMP-1, MMP-2, and MMP-9 and of the tissue inhibitors of MMPs (TIMP)-1 and TIMP-2 were semi-quantitatively determined using RT-PCR, respectively, zymography, Westernblot, and ELISA techniques and the localization of MMP-2 protein and presence of granulocytes by immunohistochemistry and Luna staining. At parturition compared to 185 days pregnancy the MMP-1 protein expression and the numbers of granulocytes were significantly increased by 3 and 26-fold respectively. MMP-2 mRNA and protein expression had already increased 2.5 (P < 0.05) and twofold (P < 0.05) at 5 days before parturition, prior to final ripening. At that time, MMP-2 was present in smooth muscle cells and extra cellular matrix. TIMP-1 mRNA expression was significantly increased at parturition and TIMP-2 mRNA expression peaked at 5 days before parturition. The increased expression of MMP-2 at 5 days before parturition, suggests that in the cow MMP-2 is responsible for collagen denaturation in the last part of gradual cervical ripening, while MMP-1 and MMP-9 are only active during the final cervical ripening process at parturition.
机译：在分娩期最终宫颈成熟之前，胶原蛋白会在怀孕后期的逐渐宫颈成熟过程中变性。基质金属蛋白酶（MMP）可能负责此过程。为了研究在成熟过程的不同阶段MMP的存在和潜在功能，在分娩时和分娩后30天从妊娠185天和275天（产犊前约5天）的10头奶牛进行了宫颈活检。使用RT-PCR分别半定量测定酶法，蛋白质印迹法，蛋白质印迹法，免疫组化和Luna染色法检测ELISA和MMP-2蛋白的定位以及粒细胞的存在。与分娩185天相比，分娩时MMP-1蛋白的表达和粒细胞的数量分别显着增加了3倍和26倍。 MMP-2 mRNA和蛋白质表达在分娩前5天，最终成熟之前已经增加了2.5（P <0.05）和两倍（P <0.05）。那时，MMP-2存在于平滑肌细胞和细胞外基质中。分娩时TIMP-1 mRNA表达显着增加，分娩前5天TIMP-2 mRNA表达达到峰值。分娩前5天MMP-2的表达增加，这表明在母牛中，MMP-2负责宫颈逐渐成熟的最后阶段的胶原变性，而MMP-1和MMP-9仅在最终宫颈成熟期间才有活性。分娩时的成熟过程。

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