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首页> 外文期刊>Molecular reproduction and development >The effect of leptin on maturing porcine oocytes is dependent on glucose concentration
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The effect of leptin on maturing porcine oocytes is dependent on glucose concentration

机译：瘦素对猪卵母细胞成熟的影响取决于葡萄糖浓度

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Increased body weight is often accompanied by increased circulating levels of leptin and glucose, which alters glucose metabolism in various tissues, including perhaps the oocyte. Alteration of glucose metabolism impacts oocyte function and may contribute to the subfertility often associated with obese individuals. The objective of this study was to determine the effect of leptin (0, 10, and 100ng/ml) on the oocyte and cumulus cells during in vitro maturation under differing glucose concentrations. We examined the effects of leptin on oocyte maturation, blastocyst development, and/or gene expression in oocytes and cumulus cells (IRS1, IGF1, PPAR, IL6, GLUT1) in a physiological glucose (2mM) and high glucose (50 mM) environment. We also evaluated the effect of leptin on glucose metabolism via glycolysis and the pentose phosphate pathway. In a physiological glucose environment, leptin did not have an influence on oocyte maturation, blastocyst development, or oocyte gene expression. Expression of GLUT1 in cumulus cells was downregulated with 100ng/ml leptin treatment, but did not affect oocyte glucose metabolism. In a high glucose environment, oocyte maturation and glycolysis were decreased, but in the presence of 100ng/ml leptin, these parameters were improved to levels similar to control. This effect is potentially mediated by an upregulation of oocyte IRS1 and a correction of cumulus cell IGF1 expression. The present study demonstrates that in a physiological glucose concentration, leptin plays a negligible role in oocyte function. However, leptin appears to modulate the deleterious impact of a high glucose environment on oocyte function. Mol. Reprod. Dev. 79: 296307, 2012
机译：体重增加通常伴随着瘦素和葡萄糖循环水平的升高，从而改变了包括卵母细胞在内的各种组织中的葡萄糖代谢。葡萄糖代谢的改变会影响卵母细胞的功能，并可能导致通常与肥胖个体有关的亚生育力。这项研究的目的是确定瘦素（0、10和100ng / ml）在不同葡萄糖浓度的体外成熟过程中对卵母细胞和卵丘细胞的影响。我们检查了瘦素对生理葡萄糖（2mM）和高葡萄糖（50 mM）环境中卵母细胞和卵丘细胞（IRS1，IGF1，PPAR，IL6，GLUT1）中卵母细胞成熟，胚泡发育和/或基因表达的影响。我们还评估了瘦素通过糖酵解和戊糖磷酸途径对葡萄糖代谢的影响。在生理葡萄糖环境中，瘦素对卵母细胞成熟，胚泡发育或卵母细胞基因表达没有影响。 GLUT1在卵丘细胞中的表达被100ng / ml瘦素处理下调，但不影响卵母细胞葡萄糖代谢。在高葡萄糖环境中，卵母细胞成熟和糖酵解降低，但是在存在100ng / ml瘦素的情况下，这些参数提高到与对照相似的水平。这种作用可能是通过卵母细胞IRS1的上调和卵丘细胞IGF1表达的校正来介导的。本研究表明，在生理葡萄糖浓度下，瘦素在卵母细胞功能中的作用可忽略不计。然而，瘦素似乎调节高葡萄糖环境对卵母细胞功能的有害影响。大声笑责备。开发人员79：296307，2012年

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