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首页> 外文期刊>Molecular reproduction and development >Oogenesis specific genes (Nobox, Oct4, Bmp15, Gdf9, Oogenesin1 and Oogenesin2) are differentially expressed during natural and gonadotropin-induced mouse follicular development
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Oogenesis specific genes (Nobox, Oct4, Bmp15, Gdf9, Oogenesin1 and Oogenesin2) are differentially expressed during natural and gonadotropin-induced mouse follicular development

机译：在自然和促性腺激素诱导的小鼠卵泡发育过程中，差异表达特定的卵子发生特定基因（Nobox，Oct4，Bmp15，Gdf9，Oogenesin1和Oogenesin2）。

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摘要

Using a semi-quantitative, single-cell sensitive RT-PCR method, we studied the expression of oogenesis specific genes (Nobox, Oct4, Bmp15, Gdf9, Oogenesin1 and Oogenesin2) in single oocytes collected from primordial, primary, secondary, preantral and antral follicles during natural and gonadotropin-induced mouse follicular development. We compared the number of transcripts of these genes, showing that they are differentially expressed, both in natural conditions and under gonadotropin-induction throughout the assessed developmental stages. Our data show a clear increase in the number of transcripts between the primordial until the preantral stages, with the exception of the Oogenesin1 transcripts under gonadotropin-induction. The number of transcripts starts decreasing at the antral stage and proceeds until the metaphase II stage, with values very similar to those obtained for the primordial oocytes in both analyzed conditions. Under exogenous gonadotropin-induction, oocyte recruitment to ovulation at the preantral stage is marked by an increase in Nobox and Oogenesin2 gene expression that is concomitant with a decrease in Oogenesin1 gene expression. Oocytes that are able to proceed into whole embryo development show a tight regulation of Nobox and Oct4 expression at the antral stage. A parallel immunocytochemical study at the protein level corroborates these findings.
机译：使用半定量，单细胞敏感的RT-PCR方法，我们研究了卵母细胞特异基因（Nobox，Oct4，Bmp15，Gdf9，Oogenesin1和Oogenesin2）在从原始，原发，继发，窦前和窦前收集的单个卵母细胞中的表达。自然和促性腺激素诱导的小鼠卵泡发育过程中的卵泡。我们比较了这些基因的转录本数量，表明它们在自然条件下和促性腺激素诱导的整个评估发育阶段均差异表达。我们的数据显示，从原始到肛门前阶段之间的转录物数量明显增加，但促性腺激素诱导下的Oogenesin1转录物除外。转录物的数量在窦房期开始减少，一直持续到中期II期，其值与两种分析条件下原始卵母细胞获得的值非常相似。在外源性促性腺激素诱导下，卵母细胞募集到卵前期以Nobox和Oogenesin2基因表达的增加为标志，同时Oogenesin1基因表达的减少。能够进入整个胚胎发育的卵母细胞在窦房期显示Nobox和Oct4表达的严格调节。在蛋白质水平上的并行免疫细胞化学研究证实了这些发现。

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