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首页> 外文期刊>Molecular reproduction and development >Global H3K9 dimethylation status is not affected by transcription, translation, or DNA replication in porcine zygotes.
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Global H3K9 dimethylation status is not affected by transcription, translation, or DNA replication in porcine zygotes.

机译：猪合子中的整体H3K9二甲基化状态不受转录，翻译或DNA复制的影响。

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摘要

Methylation of the lysine 9 residue of histone H3 (H3K9) is linked to transcriptional repression. The observed structure of chromatin in porcine and murine embryos is different with regard to H3K9 dimethylation status, leading to our hypothesis that the intracellular mechanisms responsible for H3K9 methylation would also differ between these two species. The objectives of this study were: (1) to determine the extent that DNA, mRNA, and protein synthesis serve in maintaining the asymmetrical distribution of dimethylated H3K9 in porcine zygotes, (2) determine the extent to which the intracellular localization of individual pronuclei correlated with H3K9 dimethylation status, and (3) to determine the abundance of transcripts encoding the histone methyltransferases, with H3K9 methylation activity, in porcine oocytes and embryos. Our findings are that (1) H3K9 dimethylation status is not affected by DNA replication, transcription, or protein synthesis, (2) the location of a pronucleus does not significantly affect the H3K9 dimethylation status of the chromatin within that pronucleus, and (3) the histone methyltransferases with activity for H3K9 differ in transcript abundance in porcine oocytes and cleavage stage embryos. These results support our hypothesis that there is a difference in intracellular mechanisms affecting dimethylation status of H3K9 between porcine and murine embryos.
机译：组蛋白H3（H3K9）的赖氨酸9残基的甲基化与转录抑制相关。在H3K9甲基化状态方面，在猪和鼠类胚胎中观察到的染色质结构不同，这导致我们的假设是，这两个物种之间负责H3K9甲基化的细胞内机制也将有所不同。这项研究的目的是：（1）确定DNA，mRNA和蛋白质合成在维持猪合子中二甲基化H3K9的不对称分布方面的作用，（2）确定单个核的细胞内定位相关的程度（3）确定在猪卵母细胞和胚胎中编码具有H3K9甲基化活性的组蛋白甲基转移酶的转录本的丰度。我们的发现是（1）H3K9甲基化状态不受DNA复制，转录或蛋白质合成的影响;（2）原核的位置不会显着影响该原核内染色质的H3K9二甲基化状态，以及（3）具有H3K9活性的组蛋白甲基转移酶在猪卵母细胞和卵裂期胚胎中的转录丰度不同。这些结果支持我们的假设，即在猪和鼠胚胎之间，影响H3K9的二甲基化状态的细胞内机制存在差异。

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