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首页> 外文期刊>Molecular reproduction and development >Divergent Regulation of Angiopoietin-1 and-2, Tie-2, and Thrombospondin-1 Expression by Estrogen in the Baboon Endometrium
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Divergent Regulation of Angiopoietin-1 and-2, Tie-2, and Thrombospondin-1 Expression by Estrogen in the Baboon Endometrium

机译：狒狒子宫内膜中雌激素对血管生成素-1和-2，Tie-2和血小板反应蛋白-1表达的不同调节

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Estrogen has an important role in the reconstruction of a new vascular network in the endometrium during each menstrual cycle, however, the underlying mechanisms are incompletely understood Angiopoietin-1 (Ang-1) promotes vessel assembly, whereas Ang-2 and thrombospondin-1 (TSP-1) cause vessel breakdown To determine the potential effect of estrogen on the expression of these angioregulatory factors in the endometrium, Ang-1, Ang-2, TSP-1, and Tie-2 receptor mRNA levels were assessed by real-time reverse transcriptase polymerase chain reaction in glandular epithelial and stromal cells isolated from the endometrium of ovariectomized baboons treated acutely with estradiol Corresponding protein expression was assessed by immunocytochemistry and the proximity ligation assay (PLA) during advancing stages of the baboon menstrual cycle Serum estradiol levels in ovariectomized baboons were 400 pg/ml within 4-6 hr of estradiol treatment Ang-1 mRNA levels in glandular epithelial cells increased threefold (P<0 01) within 4 hr of estradiol administration In contrast, TSP-1 mRNA levels decreased four- to fivefold (P < 0 01) in endometnal glandular epithelial and stromal cells 4-6 hr after estradiol, whereas Ang-2 and Tie-2 expression was unaltered Immunostaining for Ang-1 increased, TSP-1 decreased, and Ang-2 and Tie-2 were unaltered in the endometrium during the secretory compared with the proliferative phase of the cycle Endometrial Ang-1 protein expression, quantified by PLA, increased 10-fold (P < 0 05) between the early proliferative and late proliferative/mid-secretory phases of the menstrual cycle in association with the rise in estrogen In summary, estrogen induced a rapid, divergent, and cell-specific change in expression of angiostimulatory and angioinhibitory growth factors in the endometrium of the nonhuman primate
机译：雌激素在每个月经周期的子宫内膜新血管网络的重建中都起着重要作用，但是，其潜在机制尚未完全了解，Angiopoietin-1（Ang-1）促进血管组装，而Ang-2和thrombospondin-1（ TSP-1）引起血管破裂为了确定雌激素对子宫内膜中这些血管调节因子表达的潜在影响，通过实时评估Ang-1，Ang-2，TSP-1和Tie-2受体的mRNA水平雌二醇急性处理的经卵巢切除的狒狒子宫内膜分离的腺上皮和基质细胞中的逆转录酶聚合酶链反应通过免疫细胞化学和邻近结扎分析（PLA）评估了在狒狒月经周期进展阶段卵巢切除的血清雌二醇水平的相应蛋白表达。雌二醇处理后4-6小时内狒狒体重为400 pg / ml，腺上皮细胞Ang-1 mRNA水平升高雌二醇给药后4小时内产生三倍（P <0 01）相反，雌二醇后4-6小时，子宫内膜腺上皮和基质细胞中TSP-1 mRNA水平下降四到五倍（P <0 01），而Ang-2与周期子宫内膜Ang-1蛋白表达的增殖期相比，分泌过程中子宫内膜Ang-1的免疫染色增加，Tie-2表达不变，而TSP-1下降，Ang-2和Tie-2不变。经PLA定量，与雌激素升高有关，月经周期的早期增生期和晚期增生/分泌中期之间增加了10倍（P <0 05）。总而言之，雌激素诱导了快速，发散和细胞分裂。非人类灵长类动物子宫内膜血管刺激和血管抑制生长因子表达的特异性变化

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