...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Korean Journal of Horticultural Science & Technology >Construction of cDNA Library and EST Analysis Related to Seed-hair Characteristics in Carrot
【24h】

Construction of cDNA Library and EST Analysis Related to Seed-hair Characteristics in Carrot

机译：胡萝卜籽毛特性的cDNA文库的构建和EST分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Carrot (Daucus carota L. var. sativa) is one of the most widely used crops in the world and is nutritionally important crop. However, seed-hair which is generated in epidermal cell of seeds causes the difficulty of the seedling process, because of theseed germination and absorption inhibitions. For these reasons, carrot seeds are commercialized after mechanical hair removal process. However, in this process, various damage and seed loss occur and breeding of hairless-seed carrot cultivar is needed to overcome these various weaknesses and additional seed production costs. In this study, cDNA libraries using 2 combinations, which were composed of short-hair seed CT-ATR 615 OP 666-13 & long-hair seed CT-ATR 615 OP 671-9, and short-hair seed CT-SMR 616OP 659-1 & long-hair seed CT-SMR 616 OP 677-14, were constructed and EST sequences of each individuals were analyzed to reveal carrot seed-hair characteristics. Firstly, analyzed EST sequences were classified into FunCat functional categories. As a result, significant differences have been identified in metabolism category, protein folding and stabilization, protein binding, C-compound binding category from both of two combinations. Secondly, several candidate EST sequences related to seed trichome differentiation and cellulose biosynthetic process were selected based on GO data of EST sequences. These differences based on FunCat categories and candidate EST obtained by GO data analysis are thought to be involved in the formation of carrot seed hair.Finally, 741 SSR sites and 33 SNP sites were identified from analyzed EST sequences of two combinations. Then we designed SNP and SSR primer sets to develop molecular markers. These molecular markers will be used for classification of carrot cultivars and study seed-hair characteristic.
机译：胡萝卜（Daucus carota L. var。sativa）是世界上使用最广泛的作物之一，是重要的营养作物。然而，由于这些发芽和吸收抑制，在种子的表皮细胞中产生的种毛导致幼苗过程的困难。由于这些原因，胡萝卜种子在机械脱毛过程之后被商品化。然而，在该过程中，发生各种损害和种子损失，并且需要育种无毛种子胡萝卜品种以克服这些各种弱点和额外的种子生产成本。在这项研究中，使用2种组合的cDNA文库由短发种子CT-ATR 615 OP 666-13和长发种子CT-ATR 615 OP 671-9和短发种子CT-SMR 616OP 659组成构建了-1号和长毛种子CT-SMR 616 OP 677-14，并分析了每个个体的EST序列，以揭示胡萝卜种毛的特征。首先，将分析的EST序列分为FunCat功能类别。结果，已从两种组合中鉴定出代谢类别，蛋白质折叠和稳定，蛋白质结合，C-化合物结合类别方面的显着差异。其次，基于EST序列的GO数据，选择了与种子毛状体分化和纤维素生物合成过程相关的几种候选EST序列。通过GO数据分析获得的基于FunCat类别和候选EST的差异被认为与胡萝卜种子毛的形成有关。最后，从分析的两种组合的EST序列中鉴定出741个SSR位点和33个SNP位点。然后，我们设计了SNP和SSR引物对以开发分子标记。这些分子标记将用于胡萝卜品种的分类和研究种子-毛发特性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号