Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

Bae Yun-Ui; Ik-Jae Moon; Young-Bae Seu; Kyung-Oh Doh

首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

【24h】

Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

机译：大圆形单链有义和反义DNA鉴定与癌症相关的基因

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The single-stranded large circular (LC)-sense DNA were utilized as probes for DNA chip experiments. The microarray experiment using LC-sense DNA probes found differentially expressed genes in A549 cells as compared to WI38VA13 cells, and microarray data were well-correlated with data acquired from quantitative real-time RT-PCR. A 5K LC-sense DNA microarray was prepared, and the repeated experiments and dye swap test showed consistent expression patterns. Subsequent functional analysis using LC-antisense library of overexpressed genes identified several genes involved in A549 cell growth. These experiments demonstrated proper feature of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense microarrayand antisense libraries for an effective functional validation of genes.

机译：单链大环状（LC）敏感DNA被用作DNA芯片实验的探针。使用LC传感DNA探针的微阵列实验发现，与WI38VA13细胞相比，A549细胞中差异表达的基因，并且微阵列数据与从实时定量RT-PCR获得的数据高度相关。制备了5K LC感测DNA微阵列，重复实验和染料交换测试显示一致的表达模式。使用过表达基因的LC反义文库进行的后续功能分析确定了一些与A549细胞生长有关的基因。这些实验证明了LC正义分子作为微阵列探针DNA的适当功能，以及LC正义微阵列和反义文库结合用于基因有效功能验证的潜在实用性。

著录项

来源
《Korean Journal of Microbiology and Biotechnology》 |2010年第1期|共7页
作者
Bae Yun-Ui; Ik-Jae Moon; Young-Bae Seu; Kyung-Oh Doh;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 kor
中图分类微生物学;
关键词
Microarray; expression profiling; microarray; antisense; lung cancer;

机译：微阵列;表达谱;微阵列;反义;肺癌;

相似文献

外文文献
中文文献
专利

1. Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes [J] . Bae Yun-Ui, Ik-Jae Moon, Young-Bae Seu, Korean Journal of Microbiology and Biotechnology . 2010,第1期

机译：大圆形单链有义和反义DNA鉴定与癌症相关的基因
2. Identification of iron responsive genes by screening cDNA libraries from suppression subtractive hybridization with antisense probes from three iron conditions. [J] . Ye Z, Connor JR Nucleic Acids Research . 2000,第8期

机译：通过筛选来自三种铁条件下的反义探针的抑制性消减杂交的cDNA文库，鉴定铁反应性基因。
3. Identification of iron responsive genes by screening cDNA libraries from suppression subtractive hybridization with antisense probes from three iron conditions [J] . James R. Connor, Zheng Ye Nucleic acids research . 2000,第8期

机译：通过筛选来自三种铁条件的反义探针抑制消减杂交的cDNA文库来鉴定铁反应性基因
4. Cloning of cDNA encoding F5H from Brassica napus and regulation of lignin biosynthesis by expressing antisense gene in transgenic tobacco [C] . SHI Zhujuan, LIU Guihua, SHEN Jinxiong, Proceedings of the 12th International Rapeseed Congress: Sustainable Development in Cruciferous Oilseed Crops Production . 2007

机译：甘蓝型油菜F5H cDNA的克隆及反义基因在转基因烟草中的表达调控木质素的合成
5. Long-term inhibition of the central alpha 2B-adrenergic receptor gene via recombinant adeno-associated virus-delivered antisense DNA in hypertensive rats. [D] . Shenouda, Sherene Mary. 2005

机译：通过高血压大鼠中重组腺相关病毒传递的反义DNA长期抑制中央α2B-肾上腺素受体基因。
6. Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray [O] . Johan Vallon-Christersson, Johan Staaf, Anders Kvist, 2007

机译：常规单链cDNA微阵列检测到的非编码反义转录
7. Antisense inhibition of Bcr-Abl/c-Abl synthesis promotes telomerase activity and upregulates tankyrase in human leukemia cells11The GenBank accession numbers for human Bcr-Abl and human c-Abl cDNA sequences described in this paper are X02596/M24603 and X16416, respectively. ODN1/PS-ODN1 antisense sequences and ODN3/PS-ODN3 control sense sequences correspond to the nucleotide sites localized in the breakpoint cluster region (b3a2 junction) in the 9:22 chromosomal translocation (Philadelphia chromosome, accession numbers X02596 and M24603, version X02596.1, between ex. 3061 and 3121). ODN2/PS-ODN2 antisense sequences and ODN4/PS-ODN4 control sense sequences correspond to the nucleotide site of Abl proto-oncogene, localized between ex. 961 and 1021 (accession number X16416, version X16416.1). ODN5 and PS-ODN5 are nonsense sequences and did not show homology with both cDNAs. [O] . Bakalova Rumiana, Ohba Hideki, Zhelev Zhivko, 2004

机译：对Bcr-Abl / c-Abl合成的反义抑制可促进人白血病细胞中的端粒酶活性并上调tankase酶11本文描述的人Bcr-Abl和人c-Abl cDNA序列的GenBank登录号分别为X02596 / M24603和X16416。 ODN1 / PS-ODN1反义序列和ODN3 / PS-ODN3控制有义序列对应于9:22染色体易位（费城染色体，登录号X02596和M24603，版本X02596）中位于断点簇区域（b3a2连接）的核苷酸位点.1（例如3061和3121之间）。 ODN2 / PS-ODN2反义序列和ODN4 / PS-ODN4控制有义序列对应于Abl原癌基因的核苷酸位点，位于ex。 961和1021（登录号X16416，版本X16416.1）。 ODN5和PS-ODN5是无义序列，与两个cDNA没有同源性。
8. Identification of Genes Regulating Angiogenesis and Targeted Reduction of Antisense Therapy. [R] . Huges, C. C. 2001

机译：鉴定调节血管生成和靶向减少反义治疗的基因。

Large-Circular Single-stranded Sense and Antisense DNA for Identification of Cancer-Related Genes

摘要

著录项

相似文献

相关主题

期刊订阅