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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Anti-Oxidant Property and Inhibition of Melanin Synthesis of Eight Plant Extracts
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Anti-Oxidant Property and Inhibition of Melanin Synthesis of Eight Plant Extracts

机译：八种植物提取物的抗氧化性能和抑制黑色素的合成

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摘要

Plants extracts are good resources to find functional compounds for human health. The following eight plants were collected and total phenolic contents were determined. Acer psedo-siebolianum showed the highest phenolic contents, 16.4 mg/g, whereas Cercidiphyllum japonica showed the lowest contents, 1.9 mg/g. The DPPH free radical scavenging capacities of the plant extracts showed high activity in following order : Acer ginnala (21.3 mu g/mL) > Cornus walteri (23.9 mu g/mL) > Distylum racemosum (29.2 mu g/mL) > Castanopsis cuspidata var. Thunbergii (31.7 mu g/mL) > Acer psedo-siebolianum (34.6 mu g/mL) > Thuijopsis dolabrata cv. Aurea (53.1 mu g/mL) > Cercidiphyllum Japonica (115.2 mu g/mL). Also the mushroom tyrosinase inhibitory activities of total extracts were determined at different concentration. D. racemosum extract showed highest (49.1% at 1,000 mg) in inhibitory activity than other seven extracts. The ethanol fraction (IC50 value: 118.1 mu g/mL) from D. racemosum showed more inhibitory activity than ethyl acetate fraction (IC50 value: 203 mu g/mL). The ethanol fraction on showed no significant cytotoxicity in B16/F1 cells line up to 60 mu g/mL. Over 80 mu g/mL of ethanol fraction showed cytotoxicity in B16/F1 cells. The melanin contents of cells were significantly attenuated by ethanol fraction in a dose-dependent manner. The IC50 value of ethanol fraction was 75.4 mu g/mL.
机译：植物提取物是寻找有助于人类健康的功能性化合物的良好资源。收集以下八株植物并测定总酚含量。宏cer（Acer psedo-siebolianum）的酚含量最高，为16.4 mg / g，而日本鹿角菜的最低，为1.9 mg / g。植物提取物的DPPH自由基清除能力按以下顺序显示出高活性：金合欢（21.3μg / mL）>山茱Corn（23.9μg/ mL）>桔梗（2）μg/ mL>锥栗（Castanopsis cuspidata var） 。金枪鱼（31.7μg / mL）>枫木宏（（34.6μg / mL）> Thuijopsis dolabrata cv。 Aurea（53.1微克/毫升）> Cercidiphyllum Japonica（115.2微克/毫升）。还测定了不同浓度下总提取物的蘑菇酪氨酸酶抑制活性。消旋石extract提取物显示出最高的抑制活性（1,000 mg时为49.1％），高于其他七种提取物。消旋果蝇的乙醇级分（IC50值：118.1μg / mL）显示出比乙酸乙酯级分（IC50值：203μg / mL）更高的抑制活性。乙醇馏分在高达60μg / mL的B16 / F1细胞系中没有显示出明显的细胞毒性。超过80μg / mL的乙醇馏分在B16 / F1细胞中显示出细胞毒性。乙醇组分以剂量依赖的方式使细胞的黑色素含量显着降低。乙醇馏分的IC50值为75.4微克/毫升。

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