...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >PCR-DGGE Analysis of the Fungal Community of Red-pepper Fields Utilizing Eco-friendly Farming Methods
【24h】

PCR-DGGE Analysis of the Fungal Community of Red-pepper Fields Utilizing Eco-friendly Farming Methods

机译：利用生态耕作方法对红辣椒田地真菌群落进行PCR-DGGE分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we analyzed the changes in fungal populations of red-pepper fields employing eco-friendly farming methods, such as microbial agents and crop rotation, by using polymerase chain reactions coupled with denaturing gradient gel electrophoresis (PCR-DGGE). Primer specific for fungi were used to determine the contribution of domains to the microbial community. Analysis of planted and non-planted soil samples applying PCR-DGGE technology offered evaluation of long-term patterns in fungal species richness. To evaluate the stability of DGGE patterns from different soils, comparison of planted and non-planted soil samples were compared using PCR-DGGE. The number of DNA fragments obtained from all planted soil samples by DGGE separation was fargreater (14 to 15 bands) than that of the non-planted soil samples (3 to 4 bands). In addition, 14 bands were observed from crop continuation soil treated with agrochemicals and 18 bands from crop rotation soil treated with microbial agents. The PCR-DGGEanalysis suggests that the use of crop rotation and microbial agents benefits the fungal community more than crop continuation using agrochemicals. These results indicate that crop rotation with microbial agents was better able to support beneficial organisms, enable more effective biological control and maintain a healthier balance of nutrients, organic matter and microorganisms.
机译：在这项研究中，我们通过使用聚合酶链反应与变性梯度凝胶电泳（PCR-DGGE）结合使用生态友好的农业方法，如微生物制剂和农作物轮作，分析了红辣椒田地真菌种群的变化。真菌特异的引物用于确定结构域对微生物群落的贡献。利用PCR-DGGE技术对已种植和未种植的土壤样品进行分析，可以评估真菌物种丰富度的长期模式。为了评估不同土壤中DGGE模式的稳定性，使用PCR-DGGE比较了已种植和未种植土壤样品的比较。通过DGGE分离从所有种植的土壤样品中获得的DNA片段数量比未种植的土壤样品（3至4个波段）要大得多（14至15条带）。此外，从农用化学品处理过的作物连续土壤中观察到了14条谱带，而由微生物剂处理过的作物轮作土壤中观察到了18条谱带。 PCR-DGGE分析表明，使用农作物轮作和微生物制剂比使用农用化学品继续农作物对真菌群落的益处更大。这些结果表明，使用微生物制剂进行农作物轮作能够更好地支持有益生物，实现更有效的生物控制，并保持营养素，有机质和微生物的健康平衡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号