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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Isolation and Characterization of Burkholderia cepacia EB215, an Endophytic Bacterium Showing a Potent Antifungal Activity Against Colletotrichum Species
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Isolation and Characterization of Burkholderia cepacia EB215, an Endophytic Bacterium Showing a Potent Antifungal Activity Against Colletotrichum Species

机译：洋葱伯克霍尔德菌EB215的分离和鉴定，该细菌内生细菌对炭疽菌具有很强的抗真菌活性

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摘要

In order to develop a new microbial fungicide using endophytic bacteria for the control of anthracnoses occurring on various crops, a total of 260 bacterial strains were isolated from fresh tissues of 5 plant species. After they were cultured in brothmedium, their antifungal activities were tested for in vivo antifungal activity against cucumber anthracnose caused by Colletotrichum orbiculare. As the results, liquid cultures of 28 strains showed potent antifungal activities more than 90% against cucumber anthracnose. At 3-fold dilutions of liquid cultures, 18 strains inhibited the development of cucumber anthracnose of more than 70%. They were further tested for in vivo antifungal activity against red pepper anthracnose caused by C. coccodes and invitro antifungal activity against C. acutatum, a fungal agent causing red pepper anthracnose. Among 18 strains, a bacterial strain EB215 isolated from cucumber roots displayed the most potent antifungal activity against Colletotrichum species. It was identified as Burkholderia cepacia based on its physiological and biochemical characteristics, Biolog test and 16S rDNA gene sequence. It also controlled effectively the development of rice blast (Magnaporthe grisea), rice sheath blight (Corticium sasaki),tomato gray mold (Botrytis cinerea), and tomato late blight (Phytophthora infestans). Studies on the characterization of antifungal substances produced by B. cepacia EB215 are in progress.
机译：为了开发使用内生细菌控制各种作物上炭疽病的新型杀菌剂，从5种植物的新鲜组织中分离出总共260种细菌菌株。将它们在肉毒杆菌中培养后，测试其抗真菌活性，以检测其对球形炭疽菌引起的黄瓜炭疽病的体内抗真菌活性。结果，28个菌株的液体培养物显示出对黄瓜炭疽病的有效抗真菌活性超过90％。在3倍稀释的液体培养物中，有18种菌株抑制了超过70％的黄瓜炭疽病的发生。进一步测试了它们的体内抗真菌活性（由C. coccodes引起）对红辣椒炭疽病的抗真菌活性以及体外抗真菌活性（针对引起红辣椒炭疽病的真菌病原菌C. acutatum）。在18个菌株中，从黄瓜根中分离出的EB215细菌菌株对炭疽菌具有最强的抗真菌活性。根据其生理生化特性，Biolog试验和16S rDNA基因序列，将其鉴定为洋葱伯克霍尔德菌。它还有效地控制了稻瘟病（Magnaporthe grisea），稻鞘枯萎病（Corticium sasaki），番茄灰霉病（Botrytis cinerea）和番茄晚疫病（Phytophthora infestans）的发育。洋葱伯克霍尔德氏菌EB215产生的抗真菌物质的表征研究正在进行中。

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