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首页> 外文期刊>Korean Journal of Poultry Science >Effect of Ethylene GlycoI(EG) and Propylene Glycol(PG) on the Viability of Frozen-thawed Primordial Germ Cells(PGCs) on Korean Native Chicken(Ogye) by Vitrification
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Effect of Ethylene GlycoI(EG) and Propylene Glycol(PG) on the Viability of Frozen-thawed Primordial Germ Cells(PGCs) on Korean Native Chicken(Ogye) by Vitrification

机译：乙二醇和乙二醇对玻璃化冻融韩国原住民小鸡原始生殖细胞（PGC）活力的影响

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This study established a method for preserving chicken primordial germ cells (PGCs) that enables long-term storage in liquid nitrogen (LN2) for preservation of the species. The purpose of this study was to compare the effects of Ethylene Glycol (EG) and Propylene Glycol (PG) on viability of cryopreserved PGCs with vitrification in Korean Native Chicken (Ogye), and to fine should be find or to the optimal protocol for PGCs freezing. One of the important components of cryopreservation process is cryopreservation medium that plays a vital role in preventing cellular injury during freeze-thawing. Cryoprotective agents have been known to improve cell viability after freeze-thawing. PGCs obtained from the germinal gonade of 5.5—6 day (stage 28) chick embryos, using the MACS method were suspended in a freezing medium containing a freezing and protecting agents. Gonads were harvested from stage 28 chick embryos and pooled in groups of 10E embryos, contributing gonads to the cell suspension. The gonadal cells, including PGCs, were then frozen in 1 of the following cryoprotectant treatments: 2.5% EG, 5% EG, 10% EG, 2.5% PG, 5% PG, 10% PG, and 0% cryoprotectant as a control. Effects of exposure to vitrification solution and vitrification, with different concentrations of the cryoprotectant solution, were examined. After freezing and thawing, survival rates of the frozen-thawed PGCs from the 0, 2.5, 5, 10 and 15% EG plus FBS treatment were 44.24%, 64.51%, 85.63%, 80.51% and 73.52% (p<0.05), respectively. The viability of PGCs after freeze-thawing was significantly higher for 10% EG plus FBS treatment than for 10% PG + FBS treatment (p<0.05)(85.63% vs 66.81%). Therefore, these systems may contribute in the improvement of cryopreservation for a scarce species in birds preservation. This study established a method for preserving chicken PGC that enables systematic storage and labeling of cryopreserved PGCs in liquid N at a germplasm repository and ease of entry into a database. In the future, the importancefor this new technology is that poultry lines can be conserved while work is being conducted on improving the production of germline chimeras.
机译：这项研究建立了一种保存鸡原始生殖细胞（PGC）的方法，该方法能够在液氮（LN2）中长期保存以保护该物种。本研究的目的是比较乙二醇和丙二醇（PG）对韩国本地鸡（Ogye）中冷冻保存的PGC具有玻璃化作用的活力的影响，并应找到精确的方法或PGC的最佳方案冷冻。冷冻保存过程的重要组成部分之一是冷冻保存培养基，它在防止冻融过程中对细胞的损伤中起着至关重要的作用。已知冷冻保护剂可改善冻融后的细胞活力。使用MACS方法将从5.5-6天（第28期）雏鸡胚生殖腺获得的PGC悬浮在含有冷冻和保护剂的冷冻培养基中。从第28阶段的雏鸡胚胎中收集性腺，并汇集成一组10E胚胎，从而为细胞悬液提供性腺。然后将性腺细胞（包括PGC）冷冻在以下冷冻保护剂处理中的一种中：2.5％EG，5％EG，10％EG，2.5％PG，5％PG，10％PG和0％冷冻保护剂作为对照。检查了暴露于玻璃化溶液中和使用不同浓度的防冻剂溶液进行玻璃化的影响。冷冻和解冻后，从0％，2.5％，5％，10％和15％EG加FBS处理的冻融PGC的存活率为44.24％，64.51％，85.63％，80.51％和73.52％（p <0.05），分别。 10％EG + FBS处理的冻融后PGCs的活力显着高于10％PG + FBS处理的PGC的生存力（p <0.05）（85.63％vs 66.81％）。因此，这些系统可能有助于改善鸟类保藏中稀有物种的冷冻保存。这项研究建立了一种保存鸡PGC的方法，该方法可以在种质库中系统存储和标记液态N中的低温保存的PGC，并易于输入数据库。将来，这项新技术的重要性在于，在改进种系嵌合体生产的同时，可以保护家禽系。

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