The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter

Chinpaisal C; Chang L; Hu X; Lee CH; Wen WN; Wei LN

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The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter

机译：孤儿核受体TR2抑制小鼠CRABP-1基因启动子的DR4激素反应元件

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The mouse orphan nuclear receptor TR2-11-f suppressed the expression of reporters fused to a hormone response element of the mouse cellular retinoic acid-binding protein I gene promoter. TR2-11-f was able to bind to a direct repeat with four nucleotides in the spacer (5'TGACCTTTGGGGACCT3') located within this hormone response element as homodimers. The specificity of protein-DNA interactions was demonstrated by competition in gel retardation and antibody-mediated supershift reactions. The residues critical for TR2-11-f binding were mapped to both repeated sequences, whereas the spacer and the flanking sequences were less important. The Kd and Bmax of TR2-11-f homodimer binding to this direct repeat were determined to be 2.6 nM and 0.012 nM, respectively. By using a yeast two-hybrid system, it was demonstrated that dimerization of TR2-11-f was mediated by its ligand-binding domain. The actions of TR2-11-f in regulating cellular retinoic acid-binding protein I gene will likely influence retinoic action and availability within the cells.

机译：小鼠孤儿核受体TR2-11-f抑制了与小鼠细胞视黄酸结合蛋白I基因启动子的激素反应元件融合的报告子的表达。 TR2-11-f能够与位于此激素反应元件内的间隔子（5'TGACCTTTGGGGACCT3'）中的四个核苷酸的同型二聚体直接结合。蛋白质-DNA相互作用的特异性通过凝胶阻滞和抗体介导的超位移反应的竞争得以证明。对TR2-11-f结合至关重要的残基被定位到两个重复的序列，而间隔区和侧翼序列的重要性较小。与该直接重复结合的TR2-11-f同型二聚体的Kd和Bmax分别确定为2.6nM和0.012nM。通过使用酵母双杂交系统，证明了TR2-11-f的二聚化是由其配体结合结构域介导的。 TR2-11-f在调节细胞视黄酸结合蛋白I基因中的作用可能会影响视黄酸的作用和细胞内的可用性。

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《Biochemistry》 |1997年第46期|共8页
作者
Chinpaisal C; Chang L; Hu X; Lee CH; Wen WN; Wei LN;
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正文语种 eng
中图分类生物化学;
关键词
Animals; Base Sequence; Binding; Competitive; COS Cells; DNA-Binding Proteins metabolism; Dimerization; Gene Expression Regulation; Genes; Reporter; Ligands; Mice; Molecular Sequence Data; Nuclear Proteins metabolism; Promoter Regions (Genetics); Protein Binding; Receptors; Retinoic Acid biosynthesis genetics; Receptors; Thyroid Hormone metabolism; Recombinant Proteins biosynthesis; Research Support; Non-U.S. Gov't; Research Support; U.S. Gov't; P.H.S.; Saccharomyces cerevisiae genetics; Suppression; Genetic; Tretinoin metabolism;

机译：动物;碱基序列;结合;竞争性;COS细胞;DNA结合蛋白的代谢;二聚化;基因表达调控;基因;记者;配体;小鼠;分子序列数据;核蛋白的代谢;启动子区域（遗传学）;蛋白质结合;受体;维甲酸生物合成遗传学;受体;甲状腺激素代谢;重组蛋白生物合成;研究支持;非美国政府;研究支持;美国政府;P.H.S .;酿酒酵母遗传学;抑制;遗传;维甲酸代谢;

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专利

1. The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter [J] . Chinpaisal C, Chang L, Hu X, Biochemistry . 1997,第46期

机译：孤儿核受体TR2抑制小鼠CRABP-1基因启动子的DR4激素反应元件
2. Synergism between nuclear receptors bound to specific hormone response elements of the hepatic control region-1 and the proximal apolipoprotein C-II promoter mediate apolipoprotein C-II gene regulation by bile acids and retinoids [J] . Kardassis D., Roussou A., Papakosta P., The Biochemical Journal . 2003,第Pta2期

机译：肝脏对照区域-1的特异性激素响应元件与肝脏对照区域-1的特异性激素响应元件的协同作用和近端载脂蛋白C-II启动子通过胆汁酸和视黄醇介导载脂蛋白C-II基因调节
3. Synergism between nuclear receptors bound to specific hormone response elements of the hepatic control region-1 and the proximal apolipoprotein C-II promoter mediate apolipoprotein C-II gene regulation by bile acids and retinoids [J] . Anastasia ROUSSOU, Dimitris KARDASSIS, Iannis TALIANIDIS, The biochemical journal . 2003,第2期

机译：与肝控制区1的特定激素反应元件结合的核受体与近端载脂蛋白C-II启动子之间的协同作用通过胆汁酸和类维生素A介导载脂蛋白C-II基因调节
4. Regulation of Genes Involved in Lipid Homeostasis and the Pathogenesis of Atherosclerosis: The Role of Promoters, Enhancers, Hormone Nuclear Receptors and Auxiliary Factors in Apolipoprotein Gene Regulation [C] . Vassilis Zannis, Eleoi Zanni, Dimitris Kardassis NATO Advanced Study Institute on Vascular Endothelium : Mechanisms of Cell Signaling . 1999

机译：调节患有脂质稳态的基因和动脉粥样硬化的发病机制：启动子，增强剂，激素核受体和载脂蛋白基因调控中的辅助因子的作用
5. Positive and negative regulation of neuroendocrine gene expression by zinc finger transcription factors: Identification of a negative retinoic acid response element in the rat oxytocin promoter, and the cloning of a novel factor binding a conserved element critical for effective expression of the growth hormone gene [D] . Lipkin, Steven Monroe 1993

机译：锌指转录因子对神经内分泌基因表达的正向和负向调节：鉴定大鼠催产素启动子中的视黄酸阴性反应元件，并克隆与保守元件结合的新型因子，该元件对于生长激素基因的有效表达至关重要
6. Synergism between nuclear receptors bound to specific hormone response elements of the hepatic control region-1 and the proximal apolipoprotein C-II promoter mediate apolipoprotein C-II gene regulation by bile acids and retinoids. [O] . Dimitris Kardassis, Anastasia Roussou, Paraskevi Papakosta, 2003

机译：与肝控制区1的特定激素反应元件结合的核受体与近端载脂蛋白C-II启动子之间的协同作用通过胆汁酸和类维生素A介导载脂蛋白C-II基因调节。
7. Transcriptional Analysis of the Orphan Nuclear Receptor Constitutive Androstane Receptor (NR1I3) Gene Promoter: Identification of a Distal Glucocorticoid Response Element [O] . Jean Marc Pascussi, Maryvonne Busson-Le Coniat, Patrick Maurel, 2003

机译：孤儿核受体构成androstane受体（NR1I3）基因启动子的转录分析：远端糖皮质激素响应元件的鉴定
8. ERR Gamma: Does an Orphan Nuclear Receptor Link Steroid Hormone Biogenesis to Endocrine Resistance [R] . Riggins, R. B. 2007

机译：ERR Gamma：孤儿核受体是否将类固醇激素生物合成与内分泌抗性联系起来

The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter

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