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首页> 外文期刊>Molecular human reproduction. >Quantification of transforming growth factor beta1 (TGFbeta1) mRNA expression in mouse preimplantation embryos and determination of TGFbeta receptor (type I and type II) expression in mouse embryos and reproductive tract.
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Quantification of transforming growth factor beta1 (TGFbeta1) mRNA expression in mouse preimplantation embryos and determination of TGFbeta receptor (type I and type II) expression in mouse embryos and reproductive tract.

机译：定量分析小鼠植入前胚胎中的转化生长因子beta1（TGFbeta1）mRNA表达，并测定小鼠胚胎和生殖道中TGFbeta受体（I型和II型）的表达。

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摘要

We hypothesized that transforming growth factor beta1 (TGFbeta(1)) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGFbeta 1 mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGFbeta 1 was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 +/- 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 +/- 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 +/- 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGFbeta 1 at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGFbeta(1) type I (ALK-5) and type II TGFbeta receptors were detected in embryos from 1-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGFbeta receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGFbeta type I receptor on day 3. Expression of TGFbeta type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGFbeta(1) and that both the embryos and the reproductive tract are responsive to TGFbeta(1) in the preimplantation period.
机译：我们假设转化生长因子β1（TGFbeta（1））及其受体在植入前的胚胎和生殖道之间的相互作用中起作用。要研究此假设，使用RNA模仿剂通过竞争性逆转录聚合酶链反应对小鼠胚胎中的TGFbeta 1 mRNA进行定量。 TGFbeta 1首先在未受精的卵母细胞中检测到，受精后消失，并在2细胞阶段再次表达（4410 +/- 1330转录本/胚胎）。它的表达逐渐增加，在8细胞阶段达到高峰（58 600 +/- 17300转录物/胚），在桑ula虫阶段后迅速下降，在胚泡期达到1520 +/- 546转录物/胚的浓度。 TGFbeta 1在8细胞和桑ula虫阶段的mRNA水平显着高于其他细胞阶段（P <0.05）。还研究了TGF受体在胚胎和生殖道中的表达。 TGFbeta（1）I型（ALK-5）和II型TGFbeta受体通过免疫组织化学在从1细胞到胚泡期的胚胎中检测到。 Northern杂交和免疫组织化学显示，从妊娠的第1天到第4天，输卵管中的两种TGFbeta受体均恒定表达，而在子宫中，第3天的TGFbeta I型受体表达显着增加。在整个研究期间，子宫内的受体保持不变。这项研究表明，植入前的小鼠胚胎会产生TGFbeta（1），并且胚胎和生殖道在植入前均对TGFbeta（1）产生反应。

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