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首页> 外文期刊>Biochemistry >Synergy of in vitro thrombolytic action of combinations of recombinant staphylokinase and single-chain urokinase-type plasminogen activator
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Synergy of in vitro thrombolytic action of combinations of recombinant staphylokinase and single-chain urokinase-type plasminogen activator

机译：重组葡萄激酶和单链尿激酶型纤溶酶原激活剂联合的体外溶栓作用的协同作用

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摘要

Kinetics of lysis of human plasma clots immersed in plasma were studied in vitro at 37 degC under the influence of recombinant staphylokinase,single-chain urokinase-type plasminogen activator (scu-PA),and their simultaneous and consecutive combinations.Staphylokinase and scu-PA caused concentration-and time-dependent lysis of the clots;32 nM staphylokinase and 75 nM scu-PA separately caused 50% lysis in 4h.At these equally effective concentrations staphylokinase in 4h induced a significantly lesser exhaustion of the plasma plasminogen,alpha_2-antiplasmin,and fibrinogen than scu-PA.Combinations of staphylokinase (<30 nM) and scu-PA (<75 nM) rendered synergic thrombolytic action on the close.The synergy of thrombolytic action was more pronounced on the simultaneous addition of the two agents than on their consecutive addition,scu-PA 30 min after staphylokinase.In 4 h after the addition,staphylokinase (25 nM) or scu-PA(15nM) induced 24% and 2% lysis,respectively,whereas the simultaneous and consecutive combination of the same concentrations of these agents induced 58% and 50% lysis,respectively.The simultaneous combination of 15 nM staphylokinase and 15 nM scu-PA resultedin maximal 3.8-fold increase in the thrombolytic effect as compared to the expected total effect of the individual agents.Synergic combinations of the two agents caused lesser exhaustion of plasma plasminogen,alpha_2-antiplasmin,and fibrnogen as compared with the expected total effect of these agents used separately.Thus simultaneous and consecutive combinations of staphylokinase and scu-PA in a relatively narrow range of their concentration possessed synergistic fibrin-selective thrombolytic action on the plasma clot in vitro.
机译：在重组葡激酶，单链尿激酶型纤溶酶原激活物（scu-PA）及其同时和连续组合的影响下，在37℃下体外研究了浸入血浆的人血浆凝块的动力学。引起血凝块浓度和时间依赖性裂解; 32 nM葡萄球激酶和75 nM scu-PA在4小时内分别引起50％裂解。在这些相同有效浓度下，葡萄激酶在4小时内诱导血浆纤溶酶原，α_2-抗血浆蛋白酶的消耗明显减少。葡萄球菌激酶（<30 nM）和scu-PA（<75 nM）的组合在闭合处产生协同溶栓作用。同时添加两种药剂时溶栓作用的协同作用比在scu-PA处更强。连续添加后，sta-PA在葡萄球菌激酶后30分钟。添加后4小时，葡萄球菌激酶（25 nM）或scu-PA（15nM）分别引起24％和2％的裂解。相同浓度的这些试剂的第一种连续组合分别引起58％和50％的裂解。15 nM葡萄激酶激酶和15 nM scu-PA的同时组合导致溶栓作用比预期的总作用最大增加3.8倍与单独使用这两种药物的预期总效果相比，两种药物的增效组合导致血浆纤溶酶原，α_2-抗纤溶酶和纤维蛋白原的消耗减少。因此，葡萄球菌激酶和scu-PA的同时和连续组合它们相对较窄的浓度范围对体外血浆凝块具有协同的纤维蛋白选择性溶栓作用。

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