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首页> 外文期刊>Nano letters >A pore-cavity-pore device to trap and investigate single nanoparticles and DNA molecules in a femtoliter compartment: Confined diffusion and narrow escape
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A pore-cavity-pore device to trap and investigate single nanoparticles and DNA molecules in a femtoliter compartment: Confined diffusion and narrow escape

机译：一种用于捕获和研究飞升隔室中单个纳米颗粒和DNA分子的孔腔设备：有限的扩散和狭窄的逃逸

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摘要

Spatial confinement from the nano- to the microscale is ubiquitous in nature. Striving to understand the behavior of nanoscale objects in confined domains we present a nanofluidic silicon device which consists of two stacked nanopores forming the in/outlets to a pyramidal cavity of micrometer dimensions (10 fL volume). Being electrically addressable, charged objects can be actively loaded into, trapped inside, and unloaded from the "pore-cavity-pore" (PCP) device. When operated passively, confined Brownian motion and the entropy barriers of the nanopores govern the behavior of nano-objects within the PCP device. We present measurements with single fluorescent nanoparticles as well as particle-ensembles and analyze their trajectories and residence times. Experimental data are compared to random walk simulations and analytical theories on confined diffusion and the Brownian escape of nano-objects across entropy barriers. Single particle data corroborate analytical solutions of the narrow escape problem, but ensemble measurements indicate crowding effects even at low particle concentrations. The utilization of the device to trap biomolecules is demonstrated for single λ-DNA molecules.
机译：从纳米级到微米级的空间限制实际上是无处不在的。为了努力了解受限区域中纳米尺度物体的行为，我们提出了一种纳米流体硅器件，该器件由两个堆叠的纳米孔组成，这些纳米孔形成了微米尺寸（10 fL体积）的金字塔形腔的进出口。通过电寻址，可以将带电对象主动加载到“孔-腔-孔”（PCP）设备中，从其中捕获或从中卸载。被动操作时，受限的布朗运动和纳米孔的熵屏障控制PCP装置内纳米对象的行为。我们介绍了单个荧光纳米粒子以及粒子集合的测量结果，并分析了它们的轨迹和停留时间。将实验数据与随机行走模拟和解析理论进行了比较，该理论涉及纳米物体跨越熵屏障的局限扩散和布朗逃逸。单粒子数据证实了狭窄逃逸问题的解析解，但是整体测量表明即使在低粒子浓度下也有拥挤效应。单个λ-DNA分子已证明该装置可用于捕获生物分子。

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