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Signal-to-noise ratio enhancement of silicon nanowires biosensor with rolling circle amplification

机译：滚环放大技术增强硅纳米线生物传感器的信噪比

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摘要

Herein, we describe a novel approach for rapid, label-free and specific DNA detection by applying rolling circle amplification (RCA) based on silicon nanowire field-effect transistor (SiNW-FET) for the first time. Highly responsive SiNWs were fabricated with a complementary metal oxide semiconductor (CMOS) compatible anisotropic self-stop etching technique which eliminated the need for hybrid method. The probe DNA was immobilized on the surface of SiNW, followed by sandwich hybridization with the perfectly matched target DNA and RCA primer that acted as a primer to hybridize the RCA template. The RCA reaction created a long single-stranded DNA (ssDNA) product and thus enhanced the electronic responses of SiNW significantly. The signal-to-noise ratio (SNR) as a figure-of-merit was analyzed to estimate the signal enhancement and possible detection limit. The nanosensor showed highly sensitive concentration-dependent conductance change in response to specific target DNA sequences. Because of the binding of an abundance of repeated sequences of RCA products, the SNR of >20 for 1 fM DNA detection was achieved, implying a detection floor of 50 aM. This RCA-based SiNW biosensor also discriminated perfectly matched target DNA from one-base mismatched DNA with high selectivity due to the substantially reduced nonspecific binding onto the SiNW surface through RCA. The combination of SiNW FET sensor with RCA will increase diagnostic capacity and the ability of laboratories to detect unexpected viruses, making it a potential tool for early diagnosis of gene-related diseases.
机译：在本文中，我们首次描述了一种基于硅纳米线场效应晶体管（SiNW-FET）的滚环扩增（RCA）技术，用于快速，无标签和特异的DNA检测。使用互补金属氧化物半导体（CMOS）兼容的各向异性自停止刻蚀技术制造了高响应SiNW，从而消除了对混合方法的需求。将探针DNA固定在SiNW的表面，然后与完全匹配的目标DNA和RCA引物进行夹心杂交，RCA引物充当与RCA模板杂交的引物。 RCA反应产生了长的单链DNA（ssDNA）产物，因此显着增强了SiNW的电子响应。分析了作为品质因数的信噪比（SNR），以估计信号增强和可能的检测极限。纳米传感器响应特定的目标DNA序列显示出高度敏感的浓度依赖性电导变化。由于大量重复序列的RCA产物结合在一起，因此1 fM DNA检测的SNR达到> 20，这意味着检测底限为50 aM。这种基于RCA的SiNW生物传感器还通过高选择性将一碱基不匹配的DNA与完全匹配的靶DNA进行了区分，这是因为通过RCA大大减少了与SiNW表面的非特异性结合。 SiNW FET传感器与RCA的结合将提高诊断能力和实验室检测意外病毒的能力，使其成为早期诊断基因相关疾病的潜在工具。

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