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首页> 外文期刊>Nano letters >Oops they did it again! Carbon nanotubes hoax scientists in viability assays
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Oops they did it again! Carbon nanotubes hoax scientists in viability assays

机译：糟糕，他们又做了一次！碳纳米管欺骗科学家进行可行性分析

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New materials of emerging technological importance are single-walled carbon nanotubes (SWCNTs). Because SWCNTs will be used in commercial products in huge amounts, their effects on human health and the environment have been addressed in several studies. Inhalation studies in vivo and submerse applications in vitro have been described with diverging results. Why some indicate a strong cytotoxicity and some do not is what we report on here. Data from A549 cells incubated with carbon nanotubes fake a strong cytotoxic effect within the MTT assay after 24 h that reaches roughly 50%, whereas the same treatment with SWCNTs, but detection with WST-1, reveals no cytotoxicity. LDH, FACS-assisted mitochondrial membrane potential determination, and Annexin-V/PI staining also reveal no cytotocicity. SWCNTs appear to interact with some tetrazolium salts such as MTT but not with others (such as WST-1, INT, XTT). This interference does not seem to affect the enzymatic reaction but lies rather in the insoluble nature of MTT-formazan. Our findings strongly suggest verifying cytotoxicity data with at least two or more independent test systems for this new class of materials (nanomaterials). Moreover, we intensely recommend standardizing nanotoxicological assays with regard to the material used: there is a clear need for reference materials. MTT-formazan crystals formed in the MTT reaction are lumped with nanotubes and offer a potential mechanism to guide bioremediation and clearance for SWCNTs from "contaminated" tissue. SWCNTs are good supporting materials for tissue growth, as attachment of focal adhesions and connections to the cytoskeleton suggest.
机译：具有新兴技术重要性的新材料是单壁碳纳米管（SWCNT）。由于单壁碳纳米管将大量用于商业产品，因此其对人体健康和环境的影响已在多项研究中得到了解决。已经描述了体内吸入研究和体外潜水应用，结果各不相同。我们在这里报道的原因为何有些表明具有很强的细胞毒性而有些则没有。用碳纳米管孵育的A549细胞的数据在24小时后在MTT分析中假冒了很强的细胞毒性作用，达到约50％，而用SWCNTs进行的相同处理，但用WST-1的检测却没有细胞毒性。 LDH，FACS辅助线粒体膜电位测定和膜联蛋白V / PI染色也没有细胞毒性。 SWCNT似乎与某些四唑盐（例如MTT）相互作用，但不与其他盐（例如WST-1，INT，XTT）相互作用。这种干扰似乎并不影响酶促反应，而是在于MTT-甲酰胺的不溶性。我们的发现强烈建议使用至少两种或更多种独立的测试系统来验证这种新型材料（纳米材料）的细胞毒性数据。此外，我们强烈建议就所用材料进行标准化的纳米毒理学测定：显然需要参考材料。在MTT反应中形成的MTT-甲酰胺晶体与纳米管混在一起，并提供了一种潜在的机制来指导生物修复和从“受污染”的组织清除SWCNT。 SWCNTs是组织生长的良好支撑材料，因为粘着斑的附着和与细胞骨架的连接提示。

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