Large area protein nanopatterning for biological applications

Agheli H; Malmstrom J; Larsson EM; Textor M; Sutherland DS

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Large area protein nanopatterning for biological applications

机译：用于生物应用的大面积蛋白质纳米图案

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Large area nanopatterns of functional proteins are demonstrated. A new approach to analyze atomic force microscopy height histograms is used to quantify protein and antibody binding to nanoscale patches. Arrays of nanopatches, each containing less than 40 laminin molecules, are shown to be highly functional binding close to 1 monoclonal anti-laminin IgG (site by IKVAV sequence) or 3-4 polyclonal anti-laminin IgG's per surface bound laminin. Complementary quartz crystal microbalance measurements indicate higher functionality at nanopatches than on homogeneous surfaces.

机译：展示了功能蛋白的大面积纳米图案。分析原子力显微镜高度直方图的新方法用于量化蛋白质和抗体与纳米级贴片的结合。每个包含少于40个层粘连蛋白分子的纳米补丁阵列显示出高度功能性结合，每个表面结合的层粘连蛋白接近1个单克隆抗层粘连蛋白IgG（按IKVAV序列定位）或3-4个多克隆抗层粘连蛋白IgG。互补的石英晶体微天平测量结果表明，纳米斑片的功能性高于均质表面。

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《Nano letters》 |2006年第6期|共7页
作者
Agheli H; Malmstrom J; Larsson EM; Textor M; Sutherland DS;
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正文语种 eng
中图分类特种结构材料;
关键词
QUARTZ-CRYSTAL MICROBALANCE; SELF-ASSEMBLED MONOLAYERS; DIP-PEN NANOLITHOGRAPHY; COLLOIDAL LITHOGRAPHY; SOFT LITHOGRAPHY; NANOSPHERE LITHOGRAPHY; CHEMICAL-PATTERNS; FABRICATION; ADSORPTION; SURFACES;

机译：石英晶体微平衡;自组装单层膜;双笔纳米光刻;胶体光刻;软光刻;纳米光刻;化学图案;制造;吸附;表面;

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1. Large area protein nanopatterning for biological applications [J] . Agheli H, Malmstrom J, Larsson EM, Nano letters . 2006,第6期

机译：用于生物应用的大面积蛋白质纳米图案
2. Polymer brush nanopatterns with controllable features for protein pattern applications [J] . Yunfeng Li, Junhu Zhang, Liping Fang Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology . 2012,第48期

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3. CMC Microbiology Review of Biologics License Applications and Pre-License/Pre-Approval Inspections: Therapeutic Biological Proteins [J] . Anastasia G. Lolas, Bo Chi, Patricia F. Hughes, American pharmaceutical review . 2010,第2期

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5. Fabrication of Protein Nanopatterns for Tissue Engineering and Molecular Sensing Applications [D] . Kolodziej, Christopher Michael 2011

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6. Chemical Biology of Autophagy-Related Proteins With Posttranslational Modifications: From Chemical Synthesis to Biological Applications [O] . Yu Luo, Chen Jiang, Lihua Yu, 2020

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Large area protein nanopatterning for biological applications

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