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A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine

机译：基于DNA金纳米粒子的比色竞争法检测半胱氨酸

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摘要

We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.
机译：我们报道了基于寡核苷酸功能化的金纳米颗粒探针的半胱氨酸的高灵敏度和选择性比色检测方法的发展，该探针包含与Hg2 +复合形成的战略性放置的胸苷-胸苷（T-T）错配。该测定法依赖于金纳米颗粒的距离依赖性光学特性，寡核苷酸连接的纳米颗粒聚集体的急剧熔融转变以及Hg2 +与半胱氨酸的非常选择性的配位。半胱氨酸的浓度可通过用肉眼或紫外可见光谱仪监测与聚集体解离相关的紫红色变色发生的温度来确定。该测定法不利用有机助溶剂，酶促反应，光敏染料分子，冗长的操作流程或复杂的仪器，从而克服了更常规方法的某些局限性。

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