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Antibody responses to individual proteins of SARS coronavirus and their neutralization activities

机译：对SARS冠状病毒单个蛋白的抗体反应及其中和活性

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A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle. (c) 2005 Elsevier SAS. All rights reserved.
机译：新型冠状病毒，即严重急性呼吸系统综合症（SARS）冠状病毒（SARS-CoV），被确定为SARS的病原体。针对病毒单个蛋白的特异性抗体的概况对于疫苗和诊断工具的开发至关重要。在这项研究中，制备了与SARS-CoV的四个结构蛋白（S，E，M和N）和五个推定的未表征蛋白（3a，3b，6、7a和9b）相关的13个重组蛋白，并将其用于筛选和监测蛋白芯片检测SARS患者血清中的特异性IgG抗体。康复期SARS患者的血清中检测到了针对蛋白质S，3a，N和9b的抗体，而未检测到针对蛋白质E，M，3b，6和7a的抗体。在可检测的特异性抗体中，抗S和抗N占主导地位，并且可以在SARS患者的血清中持续存在直到30周。在兔针对重组蛋白S3，N，3a和9b的抗血清中，只有抗S3血清显示了显着性在Vero E6细胞中对SARS-CoV感染的中和活性。结果表明：（1）抗S和抗N抗体是诊断标记，特别是S3具有免疫原性，因此是亚单位疫苗抗原的良好候选者; （2）从病毒结构的角度来看，在人的血清中与两种重组多肽3a和9b反应的抗体的存在支持了在病毒周期中合成它们的假说。 （c）2005 Elsevier SAS。版权所有。

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