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首页> 外文期刊>Microbes and infection >Melanin in the dimorphic fungal pathogen Paracoccidioides brasiliensis: effects on phagocytosis, intracellular resistance and drug susceptibility.
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Melanin in the dimorphic fungal pathogen Paracoccidioides brasiliensis: effects on phagocytosis, intracellular resistance and drug susceptibility.

机译：巴西双态真菌病原体Paracoccidioides Brasiliensis中的黑色素：对吞噬作用，细胞内耐药性和药物敏感性的影响。

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摘要

The fungal pathogen Paracoccidioides brasiliensis produces a melanin-like pigment in the presence of l-DOPA in vitro. We investigated whether melanization affected yeast uptake by alveolar and peritoneal macrophages, the intracellular resistance of fungal cells and their susceptibility to antifungal drugs. The interactions of melanized and nonmelanized P. brasiliensis with murine primary macrophages and J774.16 and MH-S macrophage-like cell lines were investigated. Melanized yeast cells were poorly phagocytosed by the cells even in the presence of complement. Melanization caused significant interference with the binding of cell wall components to lectin receptors on macrophages. Melanized cells were also more resistant than nonmelanized cells to the antifungal activity of murine macrophages. No difference in the susceptibilities of melanized and nonmelanized P. brasiliensis to antifungal drugs was observed using the minimum inhibitory concentration (MIC) method. However killing assays showed that melanization significantly reduced fungal susceptibility to amphotericin B and also protected against ketoconazole, fluconazole, itraconazole and sulfamethoxazole. The present results indicate that fungal melanin protects P. brasiliensis from phagocytosis and increases its resistance to antifungal drugs.
机译：在1-DOPA存在下，真菌病原体巴西拟南芥产生黑色素样色素。我们调查了黑色素化是否会影响肺泡和腹膜巨噬细胞摄取酵母菌，真菌细胞的胞内耐药性以及它们对抗真菌药的敏感性。研究了黑色素化和非黑色素化的巴西假单胞菌与鼠原代巨噬细胞以及J774.16和MH-S巨噬细胞样细胞系的相互作用。即使在补体存在的情况下，黑色素化酵母细胞的吞噬能力也很差。黑色素化对细胞壁成分与巨噬细胞上的凝集素受体的结合产生了显着干扰。黑色素化细胞对鼠巨噬细胞的抗真菌活性也比非黑色素化细胞更强。使用最小抑菌浓度（MIC）方法，未观察到黑色素化和非黑色素化的巴西假单胞菌对抗真菌药的敏感性差异。但是，杀伤试验表明，黑色素化作用显着降低了真菌对两性霉素B的敏感性，还可以防止酮康唑，氟康唑，伊曲康唑和磺胺甲恶唑。目前的结果表明，真菌黑色素可以保护巴西假单胞菌免受吞噬作用，并增强其对抗真菌药物的抵抗力。

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