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The optical detection of individual DNA-conjugated gold nanoparticle labels after metal enhancement

机译：金属增强后单个DNA共轭金纳米颗粒标记的光学检测

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摘要

The detection of DNA using nanoparticles as labels is an interesting alternative to the standard fluorescence technique. It requires simpler detection equipment, resulting in higher stability and lower costs. Besides easier detection, metal enhancement allows a higher sensitivity of detection. The signal-response curve for labelled DNA before and after silver enhancement was studied, applying both atomic force microscope (AFM) and optical (reflection/transmission) measurements. The dynamic range and the sensitivity were determined for nanoparticle labelling with and without metal enhancement. Nanoparticle concentrations down to the fM range could be detected. The ultimate limit of detection, the identification of individual labels, is demonstrated for the optical readout. Therefore, AFM images of the particles were correlated with the optical signal of individual or clustered particles. We demonstrate that the optical signal allows the identification of single particles.
机译：使用纳米颗粒作为标记的DNA检测是标准荧光技术的一种有趣替代方法。它需要更简单的检测设备，从而具有更高的稳定性和更低的成本。除了易于检测之外，金属增强还可以提高检测灵敏度。使用原子力显微镜（AFM）和光学（反射/透射）测量方法，研究了银增强前后标记的DNA的信号响应曲线。测定具有和不具有金属增强的纳米颗粒标记的动态范围和灵敏度。可以检测到低至fM范围的纳米颗粒浓度。最终的检测极限，即单个标签的识别，被证明可以用于光学读出。因此，颗粒的AFM图像与单个或簇状颗粒的光信号相关。我们证明光信号可以识别单个粒子。

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