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首页> 外文期刊>Nanotechnology >Electrochemical immunoassay of carcinoembryonic antigen based on a lead sulfide nanoparticle label
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Electrochemical immunoassay of carcinoembryonic antigen based on a lead sulfide nanoparticle label

机译：基于硫化铅纳米粒子标记的癌胚抗原的电化学免疫测定

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摘要

We describe a lead sulfide nanoparticle (PbS NP)-based electrochemical immunoassay to detect a tumor biomarker, carcinoembryonic antigen (CEA). Cubic PbS NPs were prepared and functionalized with thioglycolic acid (TGA), which stabilized the formed NPs and offered carboxyl groups to conjugate with CEA antibodies. PbS NP conjugated with monoclonal CEA antibody was used as a label in an immunorecognition event. After a complete sandwich immunoreaction among the primary CEA antibody (immobilized on the carboxyl-modified magnetic beads), CEA and the PbS-labeled secondary antibody (PbS-anti-CEA), PbS labels were captured to the magnetic-bead (MB) surface through the antibody-antigen immunocomplex. Electrochemical stripping analysis of the captured PbS was used to quantify the concentration of CEA after an acid-dissolution step. The MBs and the magnetic separation platform were used to integrate a facile antibody immobilization with immunoreactions and the isolation of immunocomplexes from reaction solutions in the immunoassay. The voltammetric response is highly linear over the range of 1-50 ng ml(-1) CEA, and the limit of detection is estimated to be 0.5 ng ml(-1). The performance of this nanoparticle-based electrochemical immunoassay was successfully evaluated with human serum spiked with CEA, indicating that this convenient and sensitive technique offers great promise for rapid, simple and cost-effective analysis of tumor biomarkers in biological fluids.
机译：我们描述了基于硫化铅纳米粒子（PbS NP）的电化学免疫测定，以检测肿瘤生物标志物，癌胚抗原（CEA）。制备了立方PbS NP，并用巯基乙酸（TGA）功能化，从而稳定了形成的NP，并提供了羧基与CEA抗体偶联。与单克隆CEA抗体偶联的PbS NP在免疫识别事件中用作标记。在第一CEA抗体（固定在羧基修饰的磁珠上），CEA和PbS标记的第二抗体（PbS-抗CEA）之间进行完全的三明治免疫反应后，PbS标记被捕获到磁珠（MB）表面通过抗体-抗原免疫复合物。在酸溶解步骤后，对捕获的PbS进行电化学汽提分析，以定量CEA的浓度。 MBs和磁性分离平台用于整合轻便的抗体固定化和免疫反应，并在免疫测定中从反应溶液中分离出免疫复合物。伏安响应在1-50 ng ml（-1）CEA范围内是高度线性的，并且检测极限估计为0.5 ng ml（-1）。用掺有CEA的人血清成功评估了这种基于纳米颗粒的电化学免疫测定的性能，这表明这种方便而灵敏的技术为快速，简单和经济高效地分析生物流体中的肿瘤标志物提供了广阔的前景。

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