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Electrostatic force microscopy: imaging DNA and protein polarizations one by one

机译:静电力显微镜:一对一地成像DNA和蛋白质极化

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We present electrostatic force microscopy images of double-stranded DNA and transcription complex on an insulating mica substrate obtained with molecular resolution using a frequency-mode noncontact atomic force microscope. The electrostatic potential images show that both DNA and transcription complexes are polarized with an upward dipole moment. Potential differences of these molecules from the mica substrate enabled us to estimate dipole moments of isolated DNA and transcription complex in zero external field to be 0.027 D/base and 0.16 D/molecule, respectively. Scanning capacitance microscopy demonstrates characteristic contrast inversion between DNA and transcription complex images, indicating the difference in electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate while retaining complex formation.
机译:我们提出了使用频率模式非接触原子力显微镜通过分子分辨率获得的绝缘云母基底上的双链DNA和转录复合物的静电力显微镜图像。静电势图像显示DNA和转录复合物都被向上偶极矩极化。这些分子与云母底物的电位差使我们能够估计零外部场中分离的DNA和转录复合物的偶极矩分别为0.027 D /碱基和0.16 D /分子。扫描电容显微镜显示DNA和转录复合物图像之间的特征对比反转,表明这些分子的电极化率不同。这些发现表明,单个生物分子的静电特性可以在绝缘体基底上成像,同时保留复合物的形成。

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