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首页> 外文期刊>Nanotechnology >Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix
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Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix

机译：通过光子力显微镜进行细胞内纳米处理，并实时获取3D刚度矩阵

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摘要

A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditional PFM data. Our system is devoted to intracellular measurements and is fully interactive through the use of a haptic joystick. Using our specialized analog hardware along with a dedicated algorithm, we can extract the full 3D stiffness matrix of the optical trap in real time, including the off-diagonal cross-terms. Our system is also capable of simultaneously recording data for subsequent offline analysis. This allows us to check that a good correlation exists between the classical analysis of stiffness and our real-time measurements. We monitor the PFM beads using an optical microscope. The force-feedback mechanism of the haptic joystick helps us in interactively guiding the bead inside living cells and collecting information from its (possibly anisotropic) environment. The instantaneous stiffness measurements are also displayed in real time on a graphical user interface. The whole system has been built and is operational; here we present early results that confirm the consistency of the real-time measurements with offline computations.
机译：传统的光子力显微镜（PFM）会产生大量数据，这需要繁琐的数值分析。在本文中，我们提出了一种模拟信号处理器，以在保持传统PFM数据丰富性的同时获得实时功能。我们的系统专用于细胞内测量，并通过使用触觉操纵杆进行完全交互。使用我们的专用模拟硬件以及专用算法，我们可以实时提取光阱的完整3D刚度矩阵，包括非对角交叉项。我们的系统还能够同时记录数据以进行后续离线分析。这使我们能够检查经典的刚度分析与实时测量之间是否存在良好的相关性。我们使用光学显微镜监控PFM珠。触觉操纵杆的力反馈机制帮助我们以交互方式引导活细胞内部的磁珠并从其（可能是各向异性的）环境中收集信息。瞬时刚度测量值也实时显示在图形用户界面上。整个系统已经构建并可以运行;在这里，我们提供了早期结果，这些结果证实了实时测量与离线计算的一致性。

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