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Multi-walled carbon nanotubes suppress potassium channel activities in PC12 cells

机译：多壁碳纳米管抑制PC12细胞中的钾通道活性

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摘要

The advancement in nanotechnology has produced technological and conceptual breakthroughs but the effects nanomaterials have on organisms at the cellular level are poorly understood. Here we report that carboxyl-terminated multi-walled carbon nanotubes (MWCNTs) act as antagonists of three types of potassium channels as assessed by whole-cell patch clamp electrophysiology on undifferentiated pheochromocytoma (PC12) cells. Our results showed that carboxyl-terminated MWCNTs suppress the current densities ofI_(to), I_K and I_(Kl) in a time-dependent and irreversible manner. The suppressions were most distinct 24 h after incubation with MWCNTs. However, MWCNTs did not significantly change the expression levels of reactive oxygen species (ROS) or intracellular free calcium and also did not alter the mitochondrial membrane potential (ΔΨ_m) in PCl2 cells. These results suggest that oxidative stress was not involved in the MWCNTs suppression of I_(to), I_K and I_(Kl) current densities. Nonetheless, the suppression of potassium currents by MWCNTs will impact on electrical signaling of excitable cells such as neurons and muscles.
机译：纳米技术的进步已经产生了技术和概念上的突破，但是人们对纳米材料在细胞水平上对生物的影响知之甚少。在这里我们报告羧基端的多壁碳纳米管（MWCNTs）充当三种类型的钾通道的拮抗剂，通过全细胞膜片钳电生理对未分化的嗜铬细胞瘤（PC12）细胞进行评估。我们的结果表明，羧基封端的MWCNT以时间依赖性和不可逆的方式抑制了I_（to），I_K和I_（Kl）的电流密度。与MWCNTs孵育24小时后，抑制作用最为明显。然而，MWCNTs不会显着改变活性氧（ROS）或细胞内游离钙的表达水平，也不会改变PCl2细胞中的线粒体膜电位（ΔΨ_m）。这些结果表明氧化应力不参与MWCNT对I_（to），I_K和I_（Kl）电流密度的抑制。尽管如此，MWCNTs对钾电流的抑制将影响可兴奋细胞（如神经元和肌肉）的电信号传导。

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