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Microtubule bundle formation driven by ATP: the effect of concentrations of kinesin, streptavidin and microtubules

机译:ATP驱动的微管束形成:驱动蛋白,链霉亲和素和微管浓度的影响

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Recently, a method was established for the formation of microtubule (MT) assemblies by an active self-organization (AcSO) process, in which MTs were crosslinked during sliding motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate (ATP) hydrolysis. Streptavidin (ST) was the glue used to crosslink biotin-labeled MTs. Although most of the MT assemblies were in the bundle form, they varied in size, shape and motility, depending on the initial conditions used. In this paper, we systematically examined the effects of the concentrations of kinesin, ST and MT on the formation of MT bundles under the initial conditions of the process.
机译:最近,建立了一种通过主动自组织(AcSO)过程形成微管(MT)组件的方法,其中MT在驱动蛋白涂层表面上的滑动过程中发生交联,并与三磷酸腺苷(ATP)偶联。 )水解。链霉亲和素(ST)是用于交联生物素标记的MT的胶。尽管大多数MT组件呈束状,但它们的大小,形状和运动性会有所不同,具体取决于所使用的初始条件。在本文中,我们系统地研究了工艺初始条件下驱动蛋白,ST和MT的浓度对MT束形成的影响。

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